Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat

B. U. Steinmann, A Hari Reddi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The changes in rates of hydroxyproline formation and biosynthesis of types-I and -III collagen during bone-matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types-I and -III collagen at different stages of this sequence was studied by labelling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis. The results revealed that maximal amounts of type-III collagen were synthesized on day 3 during mesenchymal-cell proliferation. Thereafter, there was a gradual decline in type-III collagen synthesis. On days 9-20 during bone formation predominantly type-I collagen was synthesized. Similar results were obtained by the use of labelling techniques both in vivo and in vitro.

Original languageEnglish (US)
Pages (from-to)919-924
Number of pages6
JournalBiochemical Journal
Volume186
Issue number3
StatePublished - 1980
Externally publishedYes

Fingerprint

Collagen Type III
Collagen Type I
Rats
Bone
Collagen
Bone and Bones
Biosynthesis
Labeling
Bone Matrix
Pepsin A
Hydroxyproline
Cell proliferation
Cartilage
Electrophoresis
Proline
Osteogenesis
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Bone Marrow
Cell Proliferation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat. / Steinmann, B. U.; Reddi, A Hari.

In: Biochemical Journal, Vol. 186, No. 3, 1980, p. 919-924.

Research output: Contribution to journalArticle

@article{693a6385ae034553bbdf5ff1079d0085,
title = "Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat",
abstract = "The changes in rates of hydroxyproline formation and biosynthesis of types-I and -III collagen during bone-matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types-I and -III collagen at different stages of this sequence was studied by labelling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis. The results revealed that maximal amounts of type-III collagen were synthesized on day 3 during mesenchymal-cell proliferation. Thereafter, there was a gradual decline in type-III collagen synthesis. On days 9-20 during bone formation predominantly type-I collagen was synthesized. Similar results were obtained by the use of labelling techniques both in vivo and in vitro.",
author = "Steinmann, {B. U.} and Reddi, {A Hari}",
year = "1980",
language = "English (US)",
volume = "186",
pages = "919--924",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "3",

}

TY - JOUR

T1 - Changes in synthesis of types-I and -III collagen during matrix-induced endochondral bone differentiation in rat

AU - Steinmann, B. U.

AU - Reddi, A Hari

PY - 1980

Y1 - 1980

N2 - The changes in rates of hydroxyproline formation and biosynthesis of types-I and -III collagen during bone-matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types-I and -III collagen at different stages of this sequence was studied by labelling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis. The results revealed that maximal amounts of type-III collagen were synthesized on day 3 during mesenchymal-cell proliferation. Thereafter, there was a gradual decline in type-III collagen synthesis. On days 9-20 during bone formation predominantly type-I collagen was synthesized. Similar results were obtained by the use of labelling techniques both in vivo and in vitro.

AB - The changes in rates of hydroxyproline formation and biosynthesis of types-I and -III collagen during bone-matrix-induced sequential differentiation of cartilage, bone and bone marrow in rat were investigated. Biosynthesis of types-I and -III collagen at different stages of this sequence was studied by labelling in vivo and in vitro with [2,3-3H]proline. Pepsin-solubilized collagens were separated by sodium dodecyl sulphate/polyacrylamide-slab-gel electrophoresis. The results revealed that maximal amounts of type-III collagen were synthesized on day 3 during mesenchymal-cell proliferation. Thereafter, there was a gradual decline in type-III collagen synthesis. On days 9-20 during bone formation predominantly type-I collagen was synthesized. Similar results were obtained by the use of labelling techniques both in vivo and in vitro.

UR - http://www.scopus.com/inward/record.url?scp=0018825214&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018825214&partnerID=8YFLogxK

M3 - Article

VL - 186

SP - 919

EP - 924

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

ER -