Changes in pyrophosphatase activity during the de novo mineralization associated with cartilage and bone formation

John L. Meyer, A Hari Reddi

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Pyrophosphatase was extracted from implants undergoing de novo mineralization in an in vivo model of matrix-induced endochondral bone formation. Before the onset of the mineralization of the plaques and after the mineralization process had been completed only one form of Pyrophosphatase activity was observed. During the active deposition of calcium phosphate, however, a new, higher molecular weight form of pyrophosphatase activity was produced suggesting that this enzyme activity is associated with biological mineralization. This observation gives support to the earlier suggestion that inhibitors of calcium phosphate precipitation, such as pyrophosphate, must be removed from the site of mineralization before calcification can occur. This highmolecular-weight activity also appears to be associated with alkaline and/or acid phosphatase activity as determined by molecular exclusion chromatography.

Original languageEnglish (US)
Pages (from-to)532-539
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume242
Issue number2
DOIs
StatePublished - Nov 1 1985
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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