Changes in Proteases, Antiproteases, and Bioactive Proteins From Mother's Breast Milk to the Premature Infant Stomach

Veronique Demers-Mathieu, Søren Drud Nielsen, Mark Underwood, Robyn Borghese, David C. Dallas

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objective: Our previous studies suggested that human milk proteases begin to hydrolyze proteins in the mammary gland and continue within the term infant' stomach. No research has measured milk protease and pepsin activity in the gastric aspirates of preterm infants after human milk feeding. This study investigated how the concentrations of human milk proteases and protease inhibitors changed in the premature infant stomach. Methods: Human milk and infant gastric samples were collected from 18 preterm-delivering mother-infant pairs (24-32 week gestational age). Paired human milk and gastric samples were collected across postnatal age (2-47 days). Protease concentrations were determined by spectrophotometric or fluorometric assays, and the concentrations of protease inhibitors and bioactive proteins were determined by enzyme-linked immunosorbent assay. Paired t tests were applied to compare enzymes, antiproteases, and bioactive proteins between human milk and gastric samples. Results: Our study reveals that although human milk proteases, including carboxypeptidase B2, kallikrein, plasmin, cathepsin D, elastase, thrombin, and cytosol aminopeptidase, are present in the preterm infant stomach, only plasmin and cathepsin D can actively hydrolyze proteins at gastric pH. Enzyme-linked immunosorbent assay and peptidomic evidence suggest that all milk antiproteases as well as lactoferrin and immunoglobulin A are partially digested in the preterm stomach. Conclusions: Most human milk proteases are active in milk but not at preterm infant gastric pH. Only cathepsin D and plasmin have potential to continue degrading milk proteins within the preterm infant stomach.

Original languageEnglish (US)
Pages (from-to)318-324
Number of pages7
JournalJournal of Pediatric Gastroenterology and Nutrition
Volume66
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Human Milk
Protease Inhibitors
Premature Infants
Stomach
Peptide Hydrolases
Mothers
Proteins
Cathepsin D
Fibrinolysin
Milk
Carboxypeptidase B2
Enzyme-Linked Immunosorbent Assay
Leucyl Aminopeptidase
Kallikreins
Lactoferrin
Milk Proteins
Pepsin A
Pancreatic Elastase
Human Mammary Glands
Thrombin

Keywords

  • fluorometric assays
  • peptidomics
  • preterm milk
  • spectrophotometric assay
  • α-antitrypsin

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Gastroenterology

Cite this

Changes in Proteases, Antiproteases, and Bioactive Proteins From Mother's Breast Milk to the Premature Infant Stomach. / Demers-Mathieu, Veronique; Nielsen, Søren Drud; Underwood, Mark; Borghese, Robyn; Dallas, David C.

In: Journal of Pediatric Gastroenterology and Nutrition, Vol. 66, No. 2, 01.02.2018, p. 318-324.

Research output: Contribution to journalArticle

Demers-Mathieu, Veronique ; Nielsen, Søren Drud ; Underwood, Mark ; Borghese, Robyn ; Dallas, David C. / Changes in Proteases, Antiproteases, and Bioactive Proteins From Mother's Breast Milk to the Premature Infant Stomach. In: Journal of Pediatric Gastroenterology and Nutrition. 2018 ; Vol. 66, No. 2. pp. 318-324.
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