Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure

Hiroyuki Osawa, Hiroto Kita, Hirohide Ohnishi, Masamitsu Nakazato, Yukari Date, Christopher Bowlus, Yumiko Ishino, Eiko Watanabe, Tomomi Shiiya, Hiroaki Ueno, Hiroko Hoshino, Kiichi Satoh, Kentaro Sugano

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Background: Ghrelin is a body weight-regulating peptide produced and secreted primarily by the gastric mucosa. Helicobacter pylori infection impairs gastric ghrelin production, leading to a lower plasma ghrelin concentration. However, the effect of H. pylori eradication on plasma ghrelin levels and its relation to body weight change after H. pylori cure are still uncertain. We examined the association of plasma ghrelin levels with gastric ghrelin production and body weight change before and after H. pylori eradication. Methods: Plasma ghrelin concentrations, gastric ghrelin expression, and body weight were determined in a total of 134 consecutive individuals before and 12 weeks after successful H. pylori eradication. Gastric ghrelin expression was evaluated by determining mRNA expression levels and the number of ghrelin-producing cells in gastric mucosa biopsy specimens by real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry, respectively. Results: Plasma ghrelin concentration increased in 50 patients and decreased in 84 patients after H. pylori eradication. After H. pylori cure, however, gastric preproghrelin mRNA expression was increased nearly fourfold (P < 0.0001), and the number of ghrelin-positive cells was increased or unchanged. In contrast, plasma ghrelin changes after H. pylori cure were inversely correlated with both body weight change (P < 0.0001) and initial plasma ghrelin levels (P < 0.0001). Conclusions: Changes in plasma ghrelin concentrations before and after H. pylori cure were inversely correlated with body weight change and initial plasma ghrelin levels but not with gastric ghrelin production in Japanese patients.

Original languageEnglish (US)
Pages (from-to)954-961
Number of pages8
JournalJournal of Gastroenterology
Volume41
Issue number10
DOIs
StatePublished - Oct 2006

Fingerprint

Ghrelin
Helicobacter pylori
Stomach
Body Weight
Body Weight Changes
Gastric Mucosa
Messenger RNA
Helicobacter Infections

Keywords

  • Body weight
  • Gastric ghrelin
  • Helicobacter pylori cure
  • Plasma ghrelin

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure. / Osawa, Hiroyuki; Kita, Hiroto; Ohnishi, Hirohide; Nakazato, Masamitsu; Date, Yukari; Bowlus, Christopher; Ishino, Yumiko; Watanabe, Eiko; Shiiya, Tomomi; Ueno, Hiroaki; Hoshino, Hiroko; Satoh, Kiichi; Sugano, Kentaro.

In: Journal of Gastroenterology, Vol. 41, No. 10, 10.2006, p. 954-961.

Research output: Contribution to journalArticle

Osawa, H, Kita, H, Ohnishi, H, Nakazato, M, Date, Y, Bowlus, C, Ishino, Y, Watanabe, E, Shiiya, T, Ueno, H, Hoshino, H, Satoh, K & Sugano, K 2006, 'Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure', Journal of Gastroenterology, vol. 41, no. 10, pp. 954-961. https://doi.org/10.1007/s00535-006-1880-4
Osawa, Hiroyuki ; Kita, Hiroto ; Ohnishi, Hirohide ; Nakazato, Masamitsu ; Date, Yukari ; Bowlus, Christopher ; Ishino, Yumiko ; Watanabe, Eiko ; Shiiya, Tomomi ; Ueno, Hiroaki ; Hoshino, Hiroko ; Satoh, Kiichi ; Sugano, Kentaro. / Changes in plasma ghrelin levels, gastric ghrelin production, and body weight after Helicobacter pylori cure. In: Journal of Gastroenterology. 2006 ; Vol. 41, No. 10. pp. 954-961.
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AU - Osawa, Hiroyuki

AU - Kita, Hiroto

AU - Ohnishi, Hirohide

AU - Nakazato, Masamitsu

AU - Date, Yukari

AU - Bowlus, Christopher

AU - Ishino, Yumiko

AU - Watanabe, Eiko

AU - Shiiya, Tomomi

AU - Ueno, Hiroaki

AU - Hoshino, Hiroko

AU - Satoh, Kiichi

AU - Sugano, Kentaro

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N2 - Background: Ghrelin is a body weight-regulating peptide produced and secreted primarily by the gastric mucosa. Helicobacter pylori infection impairs gastric ghrelin production, leading to a lower plasma ghrelin concentration. However, the effect of H. pylori eradication on plasma ghrelin levels and its relation to body weight change after H. pylori cure are still uncertain. We examined the association of plasma ghrelin levels with gastric ghrelin production and body weight change before and after H. pylori eradication. Methods: Plasma ghrelin concentrations, gastric ghrelin expression, and body weight were determined in a total of 134 consecutive individuals before and 12 weeks after successful H. pylori eradication. Gastric ghrelin expression was evaluated by determining mRNA expression levels and the number of ghrelin-producing cells in gastric mucosa biopsy specimens by real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry, respectively. Results: Plasma ghrelin concentration increased in 50 patients and decreased in 84 patients after H. pylori eradication. After H. pylori cure, however, gastric preproghrelin mRNA expression was increased nearly fourfold (P < 0.0001), and the number of ghrelin-positive cells was increased or unchanged. In contrast, plasma ghrelin changes after H. pylori cure were inversely correlated with both body weight change (P < 0.0001) and initial plasma ghrelin levels (P < 0.0001). Conclusions: Changes in plasma ghrelin concentrations before and after H. pylori cure were inversely correlated with body weight change and initial plasma ghrelin levels but not with gastric ghrelin production in Japanese patients.

AB - Background: Ghrelin is a body weight-regulating peptide produced and secreted primarily by the gastric mucosa. Helicobacter pylori infection impairs gastric ghrelin production, leading to a lower plasma ghrelin concentration. However, the effect of H. pylori eradication on plasma ghrelin levels and its relation to body weight change after H. pylori cure are still uncertain. We examined the association of plasma ghrelin levels with gastric ghrelin production and body weight change before and after H. pylori eradication. Methods: Plasma ghrelin concentrations, gastric ghrelin expression, and body weight were determined in a total of 134 consecutive individuals before and 12 weeks after successful H. pylori eradication. Gastric ghrelin expression was evaluated by determining mRNA expression levels and the number of ghrelin-producing cells in gastric mucosa biopsy specimens by real-time reverse transcriptase-polymerase chain reaction and immunohistochemistry, respectively. Results: Plasma ghrelin concentration increased in 50 patients and decreased in 84 patients after H. pylori eradication. After H. pylori cure, however, gastric preproghrelin mRNA expression was increased nearly fourfold (P < 0.0001), and the number of ghrelin-positive cells was increased or unchanged. In contrast, plasma ghrelin changes after H. pylori cure were inversely correlated with both body weight change (P < 0.0001) and initial plasma ghrelin levels (P < 0.0001). Conclusions: Changes in plasma ghrelin concentrations before and after H. pylori cure were inversely correlated with body weight change and initial plasma ghrelin levels but not with gastric ghrelin production in Japanese patients.

KW - Body weight

KW - Gastric ghrelin

KW - Helicobacter pylori cure

KW - Plasma ghrelin

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