Changes in intracellular ionized Ca concentration associated with muscle fiber type transformation

F. A. Sreter, J. R. Lopez, L. Alamo, K. Mabuchi, J. Gergely

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Since increased muscle activity, which results in fast-slow fiber transformation, is associated with increases in sarcoplasmic Ca2+ concentration ([Ca2+](i)), it seemed of interest to study the level of [Ca2+] after cessation of stimulation in fibers of the extensor digitorum longus muscle chronically stimulated (8 Hz). [Ca2+](i) was measured in individual fibers with a Ca2+-sensitive electrode after subtracting the membrane potential, measured simultaneously from the potential of the Ca2+ electrode. During the first 14 days of stimulation, [Ca2+](i) increased from ~0.1 to 0.5 μM and declined in ~3 wk to a value slightly higher than the initial one. The rise and decline of [Ca2+](i) was preceded by a transient increase in total calcium. If stimulation was terminated after 7-8 wk when an essentially complete fast-to-slow transformation had taken place, a subsequent rest period led to a reverse slow-to-fast transformation, which was also preceded by a transient increase of [Ca2+](i) reaching a peak at day 5 of rest. Unstimulated fast and slow fibers and fully transformed fibers do not differ in their [Ca2+] levels; thus it appears that the transformation process itself is accompanied, particularly in its earlier stages, by elevated [Ca2+](i) levels. Elucidation of the relation between changes in Ca2+ and changes in gene expression will require further work.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume253
Issue number2
StatePublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

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