Abstract
The goal of this work is to highlight those unique aspects of contrast-enhanced diagnostic optical imaging (OI) that favor a broad clinical utilization of this emerging diagnostic technique and to illustrate certain identified challenges opposing the enthusiastic clinical welcome for the OI method. We consider the single most appealing feature of OI to be its much-touted exquisite sensitivity for the detection of near-infrared fluorescing (NIRF) probes, a sensitivity supporting the development of disease- and molecule-specific NIR diagnostic probes, akin to nuclear imaging but without the ionizing radiation and with superior spatial resolution. But a qualitative OI diagnostic examination, merely defining the presence or absence of NIRF signal, may not be sufficient. The signal must be measurable. A quantitative OI examination, capable of accurately assaying the tissue concentration of the fluorescing probe and changes in that probe concentration related to disease progression or treatment would be extremely valuable. We discuss here at least three challenges to quantitative diagnostic OI, a non-linear relationship between probe concentration and signal intensity, background signal in the form of tissue auto-fluorescence, and the requirement to define precise location and depth of the signal origin from within the subject.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of SPIE - The International Society for Optical Engineering |
| Editors | A.P. Savitsky, L.Y. Brovko, D.J. Bornhop, R. Raghavachari, S.I. Achilefu |
| Pages | 215-221 |
| Number of pages | 7 |
| Volume | 5329 |
| DOIs | |
| State | Published - 2004 |
| Externally published | Yes |
| Event | Genetically Engineered and Optical Probes for Biomedical Applications II - San Jose, CA, United States Duration: Jan 24 2004 → Jan 27 2004 |
Other
| Other | Genetically Engineered and Optical Probes for Biomedical Applications II |
|---|---|
| Country | United States |
| City | San Jose, CA |
| Period | 1/24/04 → 1/27/04 |
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Keywords
- Auto-fluorescence
- Contrast agents
- Diagnostic optical imaging
- Signal localization
- Signal quantification
ASJC Scopus subject areas
- Electrical and Electronic Engineering
- Condensed Matter Physics
Cite this
Challenges for the development of clinically-useful fluorescing diagnostic contrast agents for optical imaging : A radiologist's perspective. / Brasch, Robert C.; Fournier, Laure; Lucidi, Vincenzo; Berejnoi, Kirill; Fu, Yanjun; Palley, Jonathan; Demos, Stavros G.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / A.P. Savitsky; L.Y. Brovko; D.J. Bornhop; R. Raghavachari; S.I. Achilefu. Vol. 5329 2004. p. 215-221.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Challenges for the development of clinically-useful fluorescing diagnostic contrast agents for optical imaging
T2 - A radiologist's perspective
AU - Brasch, Robert C.
AU - Fournier, Laure
AU - Lucidi, Vincenzo
AU - Berejnoi, Kirill
AU - Fu, Yanjun
AU - Palley, Jonathan
AU - Demos, Stavros G.
PY - 2004
Y1 - 2004
N2 - The goal of this work is to highlight those unique aspects of contrast-enhanced diagnostic optical imaging (OI) that favor a broad clinical utilization of this emerging diagnostic technique and to illustrate certain identified challenges opposing the enthusiastic clinical welcome for the OI method. We consider the single most appealing feature of OI to be its much-touted exquisite sensitivity for the detection of near-infrared fluorescing (NIRF) probes, a sensitivity supporting the development of disease- and molecule-specific NIR diagnostic probes, akin to nuclear imaging but without the ionizing radiation and with superior spatial resolution. But a qualitative OI diagnostic examination, merely defining the presence or absence of NIRF signal, may not be sufficient. The signal must be measurable. A quantitative OI examination, capable of accurately assaying the tissue concentration of the fluorescing probe and changes in that probe concentration related to disease progression or treatment would be extremely valuable. We discuss here at least three challenges to quantitative diagnostic OI, a non-linear relationship between probe concentration and signal intensity, background signal in the form of tissue auto-fluorescence, and the requirement to define precise location and depth of the signal origin from within the subject.
AB - The goal of this work is to highlight those unique aspects of contrast-enhanced diagnostic optical imaging (OI) that favor a broad clinical utilization of this emerging diagnostic technique and to illustrate certain identified challenges opposing the enthusiastic clinical welcome for the OI method. We consider the single most appealing feature of OI to be its much-touted exquisite sensitivity for the detection of near-infrared fluorescing (NIRF) probes, a sensitivity supporting the development of disease- and molecule-specific NIR diagnostic probes, akin to nuclear imaging but without the ionizing radiation and with superior spatial resolution. But a qualitative OI diagnostic examination, merely defining the presence or absence of NIRF signal, may not be sufficient. The signal must be measurable. A quantitative OI examination, capable of accurately assaying the tissue concentration of the fluorescing probe and changes in that probe concentration related to disease progression or treatment would be extremely valuable. We discuss here at least three challenges to quantitative diagnostic OI, a non-linear relationship between probe concentration and signal intensity, background signal in the form of tissue auto-fluorescence, and the requirement to define precise location and depth of the signal origin from within the subject.
KW - Auto-fluorescence
KW - Contrast agents
KW - Diagnostic optical imaging
KW - Signal localization
KW - Signal quantification
UR - http://www.scopus.com/inward/record.url?scp=7644222047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=7644222047&partnerID=8YFLogxK
U2 - 10.1117/12.529648
DO - 10.1117/12.529648
M3 - Conference contribution
VL - 5329
SP - 215
EP - 221
BT - Proceedings of SPIE - The International Society for Optical Engineering
A2 - Savitsky, A.P.
A2 - Brovko, L.Y.
A2 - Bornhop, D.J.
A2 - Raghavachari, R.
A2 - Achilefu, S.I.
ER -