Abstract
The role of capsaicin-sensitive pathways and CGRP in postoperative gastric ileus was investigated. Abdominal surgery was performed under enflurane anesthesia, and 5 min later, the 20-min rate of gastric emptying was measured by the phenol red method in conscious rats. Surgery inhibited gastric emptying by 76-83% compared with rats receiving anesthesia alone. Capsaicin on the celiac/mesenteric ganglia (10-21 days before) reduced gastric ileus by 33 ± 8%, whereas perivagal capsaicin had no effect. The IV CGRP-induced inhibition of gastric emptying was completely reversed by the CGRP antagonist, CGRP(8-37) (30 μg, IV); CGRP(8-37) (15, 30, or 60 μg) or CGRP monoclonal antibody #4901 (2 mg protein) decreased the inhibition of gastric emptying by 11 ± 7%, 51 ± 13%, 47 ± 3%, and 45 ± 17%, respectively. These results indicate that CGRP and splanchnic capsaicin-sensitive afferents are involved in mediating part of the gastric ileus observed immediately after abdominal surgery.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1225-1229 |
| Number of pages | 5 |
| Journal | Peptides |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| State | Published - 1993 |
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Keywords
- Abdominal surgery
- Capsaicin
- CCK
- CGRP antagonist
- CGRP antibody
- MK-329
- Splanchnic afferents
- Substance P
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Physiology
- Cellular and Molecular Neuroscience
Cite this
CGRP antagonists and capsaicin on celiac ganglia partly prevent postoperative gastric ileus. / Plourde, Victor; Wong, Helen C.; Walsh, John H.; Raybould, Helen E; Taché, Yvette.
In: Peptides, Vol. 14, No. 6, 1993, p. 1225-1229.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CGRP antagonists and capsaicin on celiac ganglia partly prevent postoperative gastric ileus
AU - Plourde, Victor
AU - Wong, Helen C.
AU - Walsh, John H.
AU - Raybould, Helen E
AU - Taché, Yvette
PY - 1993
Y1 - 1993
N2 - The role of capsaicin-sensitive pathways and CGRP in postoperative gastric ileus was investigated. Abdominal surgery was performed under enflurane anesthesia, and 5 min later, the 20-min rate of gastric emptying was measured by the phenol red method in conscious rats. Surgery inhibited gastric emptying by 76-83% compared with rats receiving anesthesia alone. Capsaicin on the celiac/mesenteric ganglia (10-21 days before) reduced gastric ileus by 33 ± 8%, whereas perivagal capsaicin had no effect. The IV CGRP-induced inhibition of gastric emptying was completely reversed by the CGRP antagonist, CGRP(8-37) (30 μg, IV); CGRP(8-37) (15, 30, or 60 μg) or CGRP monoclonal antibody #4901 (2 mg protein) decreased the inhibition of gastric emptying by 11 ± 7%, 51 ± 13%, 47 ± 3%, and 45 ± 17%, respectively. These results indicate that CGRP and splanchnic capsaicin-sensitive afferents are involved in mediating part of the gastric ileus observed immediately after abdominal surgery.
AB - The role of capsaicin-sensitive pathways and CGRP in postoperative gastric ileus was investigated. Abdominal surgery was performed under enflurane anesthesia, and 5 min later, the 20-min rate of gastric emptying was measured by the phenol red method in conscious rats. Surgery inhibited gastric emptying by 76-83% compared with rats receiving anesthesia alone. Capsaicin on the celiac/mesenteric ganglia (10-21 days before) reduced gastric ileus by 33 ± 8%, whereas perivagal capsaicin had no effect. The IV CGRP-induced inhibition of gastric emptying was completely reversed by the CGRP antagonist, CGRP(8-37) (30 μg, IV); CGRP(8-37) (15, 30, or 60 μg) or CGRP monoclonal antibody #4901 (2 mg protein) decreased the inhibition of gastric emptying by 11 ± 7%, 51 ± 13%, 47 ± 3%, and 45 ± 17%, respectively. These results indicate that CGRP and splanchnic capsaicin-sensitive afferents are involved in mediating part of the gastric ileus observed immediately after abdominal surgery.
KW - Abdominal surgery
KW - Capsaicin
KW - CCK
KW - CGRP antagonist
KW - CGRP antibody
KW - MK-329
KW - Splanchnic afferents
KW - Substance P
UR - http://www.scopus.com/inward/record.url?scp=0027376581&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027376581&partnerID=8YFLogxK
U2 - 10.1016/0196-9781(93)90180-O
DO - 10.1016/0196-9781(93)90180-O
M3 - Article
C2 - 7510881
AN - SCOPUS:0027376581
VL - 14
SP - 1225
EP - 1229
JO - Peptides
JF - Peptides
SN - 0196-9781
IS - 6
ER -