cGMP is decreased after acute ischemia in chronically ischemic canine limbs

M. T. Eginton, B. W. Mays, H. Kelley, C. D. Hower, M. Dassow, R. A. Cambria, J. B. Towne, G. R. Seabrook, J. A. Freischlag

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background. A chronic partially ischemic state may alter the skeletal muscle response to acute ischemia and free radical formation. Methods. In order to investigate this hypothesis, a chronic ischemic state was established by ligating the right femoral artery of four mongrel dogs. ABIs were decreased from 1.05 ± 0.25 preligation to 0.54 ± 0.14 at 6 weeks (P = 0.04). At the end of 8 weeks, the hindlimb was subjected to 3 h of acute ischemia by clamping the iliac artery. The clamp was then released for 2 h of reperfusion. Plasma samples from the right iliac vein were taken during the ischemia-reperfusion period for analysis of cGMP. Tibialis anterior biopsies for Western analysis of eNOS and iNOS were taken upon completion of reperfusion. Comparisons to control dogs subjected to the acute ischemia and reperfusion without prior femoral artery ligation were made. Results. cGMP levels were increased in the controls at 3 h of ischemia (3539 ± 350) and 2 h of reperfusion (2880 ± 269). The chronic ischemia group did not develop a corresponding increase in cGMP at 3 h of ischemia (2762 ± 251) or after 2 h of reperfusion (2102 ± 130). Western analysis of eNOS and iNOS revealed similar levels in both groups. Analysis of eNOS revealed 0.6429 ± 0.086 and 0.5916 ± 0.072 (densitometric units ± SEM) for study and control dogs, respectively. Analysis of iNOS revealed 0.3401 ± 0.067 and 0.2475 ± 0.066 for study and control dogs, respectively. Conclusion. Previous ligation of the femoral artery resulting in chronic partial ischemia in this model demonstrated no increase in cGMP following acute ischemia that was not accompanied by a change in eNOS or iNOS levels. Nitric oxide activity is reflected by cGMP levels, which may increase in response to free radicals in the acute setting of complete ischemia.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalJournal of Surgical Research
Volume86
Issue number2
DOIs
StatePublished - Oct 1999
Externally publishedYes

Fingerprint

Canidae
Ischemia
Extremities
Reperfusion
Femoral Artery
Dogs
Free Radicals
Ligation
Iliac Vein
Iliac Artery
Hindlimb
Constriction
Nitric Oxide
Skeletal Muscle
Demography
Biopsy

Keywords

  • Chronic ischemia
  • Cyclic GMP
  • Ischemia
  • Nitric oxide
  • Nitric oxide synthase
  • Reperfusion injury

ASJC Scopus subject areas

  • Surgery

Cite this

Eginton, M. T., Mays, B. W., Kelley, H., Hower, C. D., Dassow, M., Cambria, R. A., ... Freischlag, J. A. (1999). cGMP is decreased after acute ischemia in chronically ischemic canine limbs. Journal of Surgical Research, 86(2), 167-170. https://doi.org/10.1006/jsre.1999.5712

cGMP is decreased after acute ischemia in chronically ischemic canine limbs. / Eginton, M. T.; Mays, B. W.; Kelley, H.; Hower, C. D.; Dassow, M.; Cambria, R. A.; Towne, J. B.; Seabrook, G. R.; Freischlag, J. A.

In: Journal of Surgical Research, Vol. 86, No. 2, 10.1999, p. 167-170.

Research output: Contribution to journalArticle

Eginton, MT, Mays, BW, Kelley, H, Hower, CD, Dassow, M, Cambria, RA, Towne, JB, Seabrook, GR & Freischlag, JA 1999, 'cGMP is decreased after acute ischemia in chronically ischemic canine limbs', Journal of Surgical Research, vol. 86, no. 2, pp. 167-170. https://doi.org/10.1006/jsre.1999.5712
Eginton MT, Mays BW, Kelley H, Hower CD, Dassow M, Cambria RA et al. cGMP is decreased after acute ischemia in chronically ischemic canine limbs. Journal of Surgical Research. 1999 Oct;86(2):167-170. https://doi.org/10.1006/jsre.1999.5712
Eginton, M. T. ; Mays, B. W. ; Kelley, H. ; Hower, C. D. ; Dassow, M. ; Cambria, R. A. ; Towne, J. B. ; Seabrook, G. R. ; Freischlag, J. A. / cGMP is decreased after acute ischemia in chronically ischemic canine limbs. In: Journal of Surgical Research. 1999 ; Vol. 86, No. 2. pp. 167-170.
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abstract = "Background. A chronic partially ischemic state may alter the skeletal muscle response to acute ischemia and free radical formation. Methods. In order to investigate this hypothesis, a chronic ischemic state was established by ligating the right femoral artery of four mongrel dogs. ABIs were decreased from 1.05 ± 0.25 preligation to 0.54 ± 0.14 at 6 weeks (P = 0.04). At the end of 8 weeks, the hindlimb was subjected to 3 h of acute ischemia by clamping the iliac artery. The clamp was then released for 2 h of reperfusion. Plasma samples from the right iliac vein were taken during the ischemia-reperfusion period for analysis of cGMP. Tibialis anterior biopsies for Western analysis of eNOS and iNOS were taken upon completion of reperfusion. Comparisons to control dogs subjected to the acute ischemia and reperfusion without prior femoral artery ligation were made. Results. cGMP levels were increased in the controls at 3 h of ischemia (3539 ± 350) and 2 h of reperfusion (2880 ± 269). The chronic ischemia group did not develop a corresponding increase in cGMP at 3 h of ischemia (2762 ± 251) or after 2 h of reperfusion (2102 ± 130). Western analysis of eNOS and iNOS revealed similar levels in both groups. Analysis of eNOS revealed 0.6429 ± 0.086 and 0.5916 ± 0.072 (densitometric units ± SEM) for study and control dogs, respectively. Analysis of iNOS revealed 0.3401 ± 0.067 and 0.2475 ± 0.066 for study and control dogs, respectively. Conclusion. Previous ligation of the femoral artery resulting in chronic partial ischemia in this model demonstrated no increase in cGMP following acute ischemia that was not accompanied by a change in eNOS or iNOS levels. Nitric oxide activity is reflected by cGMP levels, which may increase in response to free radicals in the acute setting of complete ischemia.",
keywords = "Chronic ischemia, Cyclic GMP, Ischemia, Nitric oxide, Nitric oxide synthase, Reperfusion injury",
author = "Eginton, {M. T.} and Mays, {B. W.} and H. Kelley and Hower, {C. D.} and M. Dassow and Cambria, {R. A.} and Towne, {J. B.} and Seabrook, {G. R.} and Freischlag, {J. A.}",
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T1 - cGMP is decreased after acute ischemia in chronically ischemic canine limbs

AU - Eginton, M. T.

AU - Mays, B. W.

AU - Kelley, H.

AU - Hower, C. D.

AU - Dassow, M.

AU - Cambria, R. A.

AU - Towne, J. B.

AU - Seabrook, G. R.

AU - Freischlag, J. A.

PY - 1999/10

Y1 - 1999/10

N2 - Background. A chronic partially ischemic state may alter the skeletal muscle response to acute ischemia and free radical formation. Methods. In order to investigate this hypothesis, a chronic ischemic state was established by ligating the right femoral artery of four mongrel dogs. ABIs were decreased from 1.05 ± 0.25 preligation to 0.54 ± 0.14 at 6 weeks (P = 0.04). At the end of 8 weeks, the hindlimb was subjected to 3 h of acute ischemia by clamping the iliac artery. The clamp was then released for 2 h of reperfusion. Plasma samples from the right iliac vein were taken during the ischemia-reperfusion period for analysis of cGMP. Tibialis anterior biopsies for Western analysis of eNOS and iNOS were taken upon completion of reperfusion. Comparisons to control dogs subjected to the acute ischemia and reperfusion without prior femoral artery ligation were made. Results. cGMP levels were increased in the controls at 3 h of ischemia (3539 ± 350) and 2 h of reperfusion (2880 ± 269). The chronic ischemia group did not develop a corresponding increase in cGMP at 3 h of ischemia (2762 ± 251) or after 2 h of reperfusion (2102 ± 130). Western analysis of eNOS and iNOS revealed similar levels in both groups. Analysis of eNOS revealed 0.6429 ± 0.086 and 0.5916 ± 0.072 (densitometric units ± SEM) for study and control dogs, respectively. Analysis of iNOS revealed 0.3401 ± 0.067 and 0.2475 ± 0.066 for study and control dogs, respectively. Conclusion. Previous ligation of the femoral artery resulting in chronic partial ischemia in this model demonstrated no increase in cGMP following acute ischemia that was not accompanied by a change in eNOS or iNOS levels. Nitric oxide activity is reflected by cGMP levels, which may increase in response to free radicals in the acute setting of complete ischemia.

AB - Background. A chronic partially ischemic state may alter the skeletal muscle response to acute ischemia and free radical formation. Methods. In order to investigate this hypothesis, a chronic ischemic state was established by ligating the right femoral artery of four mongrel dogs. ABIs were decreased from 1.05 ± 0.25 preligation to 0.54 ± 0.14 at 6 weeks (P = 0.04). At the end of 8 weeks, the hindlimb was subjected to 3 h of acute ischemia by clamping the iliac artery. The clamp was then released for 2 h of reperfusion. Plasma samples from the right iliac vein were taken during the ischemia-reperfusion period for analysis of cGMP. Tibialis anterior biopsies for Western analysis of eNOS and iNOS were taken upon completion of reperfusion. Comparisons to control dogs subjected to the acute ischemia and reperfusion without prior femoral artery ligation were made. Results. cGMP levels were increased in the controls at 3 h of ischemia (3539 ± 350) and 2 h of reperfusion (2880 ± 269). The chronic ischemia group did not develop a corresponding increase in cGMP at 3 h of ischemia (2762 ± 251) or after 2 h of reperfusion (2102 ± 130). Western analysis of eNOS and iNOS revealed similar levels in both groups. Analysis of eNOS revealed 0.6429 ± 0.086 and 0.5916 ± 0.072 (densitometric units ± SEM) for study and control dogs, respectively. Analysis of iNOS revealed 0.3401 ± 0.067 and 0.2475 ± 0.066 for study and control dogs, respectively. Conclusion. Previous ligation of the femoral artery resulting in chronic partial ischemia in this model demonstrated no increase in cGMP following acute ischemia that was not accompanied by a change in eNOS or iNOS levels. Nitric oxide activity is reflected by cGMP levels, which may increase in response to free radicals in the acute setting of complete ischemia.

KW - Chronic ischemia

KW - Cyclic GMP

KW - Ischemia

KW - Nitric oxide

KW - Nitric oxide synthase

KW - Reperfusion injury

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