CESA TRAFFICKING INHIBITOR inhibits cellulose deposition and interferes with the trafficking of cellulose synthase complexes and their associated proteins KORRIGAN1 and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1

Natasha Worden, Thomas E. Wilkop, Victor Esteva Esteve, Richard Jeannotte, Rahul Lathe, Samantha Vernhettes, Bart C Weimer, Glenn Hicks, Jose Alonso, John Labavitch, Staffan Persson, David Ehrhardt, Georgia Drakakaki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Cellulose synthase complexes (CSCs) at the plasma membrane (PM) are aligned with cortical microtubules (MTs) and direct the biosynthesis of cellulose. The mechanism of the interaction between CSCs and MTs, and the cellular determinants that control the delivery of CSCs at the PM, are not yet well understood. We identified a unique small molecule, CESA TRAFFICKING INHIBITOR (CESTRIN), which reduces cellulose content and alters the anisotropic growth of Arabidopsis (Arabidopsis thaliana) hypocotyls. We monitored the distribution and mobility of fluorescently labeled cellulose synthases (CESAs) in live Arabidopsis cells under chemical exposure to characterize their subcellular effects. CESTRIN reduces the velocity of PM CSCs and causes their accumulation in the cell cortex. The CSC-associated proteins KORRIGAN1 (KOR1) and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1 (CSI1) were differentially affected by CESTRIN treatment, indicating different forms of association with the PM CSCs. KOR1 accumulated in bodies similar to CESA; however, POM2/CSI1 dissociated into the cytoplasm. In addition, MT stability was altered without direct inhibition of MT polymerization, suggesting a feedback mechanism caused by cellulose interference. The selectivity of CESTRIN was assessed using a variety of subcellular markers for which nomorphological effectwas observed. The association of CESAs with vesicles decorated by the trans-Golgi network-localized protein SYNTAXIN OF PLANTS61 (SYP61) was increased under CESTRIN treatment, implicating SYP61 compartments in CESA trafficking. The properties of CESTRIN compared with known CESA inhibitors afford unique avenues to study and understand the mechanism under which PM-associated CSCs are maintained and interact with MTs and to dissect their trafficking routes in etiolated hypocotyls.

Original languageEnglish (US)
Pages (from-to)381-393
Number of pages13
JournalPlant Physiology
Volume167
Issue number2
DOIs
StatePublished - 2015

Fingerprint

cellulose synthase
Cellulose
cellulose
Proteins
proteins
Microtubules
microtubules
plasma membrane
Cell Membrane
Arabidopsis
Hypocotyl
hypocotyls
trans-Golgi Network

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

CESA TRAFFICKING INHIBITOR inhibits cellulose deposition and interferes with the trafficking of cellulose synthase complexes and their associated proteins KORRIGAN1 and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1. / Worden, Natasha; Wilkop, Thomas E.; Esteve, Victor Esteva; Jeannotte, Richard; Lathe, Rahul; Vernhettes, Samantha; Weimer, Bart C; Hicks, Glenn; Alonso, Jose; Labavitch, John; Persson, Staffan; Ehrhardt, David; Drakakaki, Georgia.

In: Plant Physiology, Vol. 167, No. 2, 2015, p. 381-393.

Research output: Contribution to journalArticle

Worden, N, Wilkop, TE, Esteve, VE, Jeannotte, R, Lathe, R, Vernhettes, S, Weimer, BC, Hicks, G, Alonso, J, Labavitch, J, Persson, S, Ehrhardt, D & Drakakaki, G 2015, 'CESA TRAFFICKING INHIBITOR inhibits cellulose deposition and interferes with the trafficking of cellulose synthase complexes and their associated proteins KORRIGAN1 and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1', Plant Physiology, vol. 167, no. 2, pp. 381-393. https://doi.org/10.1104/pp.114.249003
Worden, Natasha ; Wilkop, Thomas E. ; Esteve, Victor Esteva ; Jeannotte, Richard ; Lathe, Rahul ; Vernhettes, Samantha ; Weimer, Bart C ; Hicks, Glenn ; Alonso, Jose ; Labavitch, John ; Persson, Staffan ; Ehrhardt, David ; Drakakaki, Georgia. / CESA TRAFFICKING INHIBITOR inhibits cellulose deposition and interferes with the trafficking of cellulose synthase complexes and their associated proteins KORRIGAN1 and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1. In: Plant Physiology. 2015 ; Vol. 167, No. 2. pp. 381-393.
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abstract = "Cellulose synthase complexes (CSCs) at the plasma membrane (PM) are aligned with cortical microtubules (MTs) and direct the biosynthesis of cellulose. The mechanism of the interaction between CSCs and MTs, and the cellular determinants that control the delivery of CSCs at the PM, are not yet well understood. We identified a unique small molecule, CESA TRAFFICKING INHIBITOR (CESTRIN), which reduces cellulose content and alters the anisotropic growth of Arabidopsis (Arabidopsis thaliana) hypocotyls. We monitored the distribution and mobility of fluorescently labeled cellulose synthases (CESAs) in live Arabidopsis cells under chemical exposure to characterize their subcellular effects. CESTRIN reduces the velocity of PM CSCs and causes their accumulation in the cell cortex. The CSC-associated proteins KORRIGAN1 (KOR1) and POM2/CELLULOSE SYNTHASE INTERACTIVE PROTEIN1 (CSI1) were differentially affected by CESTRIN treatment, indicating different forms of association with the PM CSCs. KOR1 accumulated in bodies similar to CESA; however, POM2/CSI1 dissociated into the cytoplasm. In addition, MT stability was altered without direct inhibition of MT polymerization, suggesting a feedback mechanism caused by cellulose interference. The selectivity of CESTRIN was assessed using a variety of subcellular markers for which nomorphological effectwas observed. The association of CESAs with vesicles decorated by the trans-Golgi network-localized protein SYNTAXIN OF PLANTS61 (SYP61) was increased under CESTRIN treatment, implicating SYP61 compartments in CESA trafficking. The properties of CESTRIN compared with known CESA inhibitors afford unique avenues to study and understand the mechanism under which PM-associated CSCs are maintained and interact with MTs and to dissect their trafficking routes in etiolated hypocotyls.",
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AU - Worden, Natasha

AU - Wilkop, Thomas E.

AU - Esteve, Victor Esteva

AU - Jeannotte, Richard

AU - Lathe, Rahul

AU - Vernhettes, Samantha

AU - Weimer, Bart C

AU - Hicks, Glenn

AU - Alonso, Jose

AU - Labavitch, John

AU - Persson, Staffan

AU - Ehrhardt, David

AU - Drakakaki, Georgia

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