Cervicovaginal evaluation in macaques used as a model for topical microbicide safety studies

Diana G. Scorpio, Dawn S. Ruben, Zhaohao Liao, James Hildreth, Craig A. Fletcher

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Introduction: Macaques are a commonly used non-human primate (NHP) model to evaluate safety and efficacy of topically applied vaginal microbicides. Cervicovaginal evaluation for topical microbicide safety studies requires proper technique, equipment, supplies, and sequence of sample collection. Materiala and methods: Eleven rhesus macaques received a comprehensive sequential cervicovaginal examination under sedation before treatment and 24 hours post-instillation of test material. Examination was initiated with colposcopy, followed by diagnostics including vaginal culture, pH determination, cervicovaginal lavage, and cervicovaginal biopsy. Results: Overall, the methods performed yielded samples that were appropriate for diagnostic evaluation and interpretation, and the macaques experienced minimal discomfort and complications. Discussion: This paper provides a descriptive summary of compiled techniques required to conduct a safety evaluation for topically applied vaginal microbicides. This novel method-based approach should be methodically executed when evaluating a vaginally-applied, topical microbicide candidate.

Original languageEnglish (US)
Pages (from-to)65-73
Number of pages9
JournalJournal of Medical Primatology
Volume37
Issue numberSUPPL.1
DOIs
StatePublished - Feb 2008
Externally publishedYes

Fingerprint

Local Anti-Infective Agents
Macaca
anti-infective agents
Anti-Infective Agents
Safety
Colposcopy
Therapeutic Irrigation
Macaca mulatta
Primates
colposcopy
methodology
Biopsy
sedation
Equipment and Supplies
biopsy
animal models
sampling
Therapeutics
testing

Keywords

  • Cervix
  • Colposcopy
  • Non-human primate
  • Rhesus
  • Vaginal pH

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Cervicovaginal evaluation in macaques used as a model for topical microbicide safety studies. / Scorpio, Diana G.; Ruben, Dawn S.; Liao, Zhaohao; Hildreth, James; Fletcher, Craig A.

In: Journal of Medical Primatology, Vol. 37, No. SUPPL.1, 02.2008, p. 65-73.

Research output: Contribution to journalArticle

Scorpio, Diana G. ; Ruben, Dawn S. ; Liao, Zhaohao ; Hildreth, James ; Fletcher, Craig A. / Cervicovaginal evaluation in macaques used as a model for topical microbicide safety studies. In: Journal of Medical Primatology. 2008 ; Vol. 37, No. SUPPL.1. pp. 65-73.
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