Cerebral white matter contains PDGF-responsive precursors to O2A cells

J. B. Grinspan, J. L. Stern, S. M. Pustilnik, David E Pleasure

Research output: Contribution to journalArticle

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Abstract

Cells dissociated from the cerebral white matter of immature rats were maintained in monolayer culture. Treatment with platelet-derived growth factor (PDGF) caused a large increase in the numbers of "O2A" oligodendroglial precursor cells (which bind the monoclonal antibody A2B5) and subsequently in the numbers of galactocerebroside (galC)-positive oligodendroglia. A2B5-negative "pre-O2A cells" in cerebral white matter cultures in which O2A cells and oligodendroglia had been killed by antibody-dependent complement-mediated cytolysis were induced by PDGF to proliferate and to differentiate into O2A cells and subsequently into oligodendroglia and type 2 astroglia. The most mature pre-O2A phenotype in these cultures was a small, round, process-bearing cell which expressed vimentin but not glial fibrillary acidic protein or galC. Cells of this phenotype were not observed upon PDGF treatment of immature rat optic nerve monolayer cultures from which O2A cells and oligodendrocytes had been depleted, and PDGF also failed to elicit the accumulation of O2A cells and oligodendroglia in such cultures.

Original languageEnglish (US)
Pages (from-to)1866-1873
Number of pages8
JournalJournal of Neuroscience
Volume10
Issue number6
StatePublished - 1990
Externally publishedYes

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Platelet-Derived Growth Factor
Oligodendroglia
Phenotype
White Matter
Glial Fibrillary Acidic Protein
Vimentin
Optic Nerve
Astrocytes
Monoclonal Antibodies
Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Grinspan, J. B., Stern, J. L., Pustilnik, S. M., & Pleasure, D. E. (1990). Cerebral white matter contains PDGF-responsive precursors to O2A cells. Journal of Neuroscience, 10(6), 1866-1873.

Cerebral white matter contains PDGF-responsive precursors to O2A cells. / Grinspan, J. B.; Stern, J. L.; Pustilnik, S. M.; Pleasure, David E.

In: Journal of Neuroscience, Vol. 10, No. 6, 1990, p. 1866-1873.

Research output: Contribution to journalArticle

Grinspan, JB, Stern, JL, Pustilnik, SM & Pleasure, DE 1990, 'Cerebral white matter contains PDGF-responsive precursors to O2A cells', Journal of Neuroscience, vol. 10, no. 6, pp. 1866-1873.
Grinspan, J. B. ; Stern, J. L. ; Pustilnik, S. M. ; Pleasure, David E. / Cerebral white matter contains PDGF-responsive precursors to O2A cells. In: Journal of Neuroscience. 1990 ; Vol. 10, No. 6. pp. 1866-1873.
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