Abstract
A method of measuring cortical oxygen metabolism in the mouse brain that uses independent quantitative measurements of three key parameters: cerebral blood flow (CBF), arteriovenous oxygen extraction (OE), and hemoglobin concentration ([HbT]) is presented. Measurements were performed using a single visible light spectral/Fourier domain OCT microscope, with Doppler and spectroscopic capabilities, through a thinnedskull cranial window in the mouse brain. Baseline metabolic measurements in mice are shown to be consistent with literature values. Oxygen consumption, as measured by this method, did not change substantially during minor changes either in the fraction of inspired oxygen (FiO2) or in the fraction of inspired carbon dioxide (FiCO2), in spite of larger variations in oxygen saturations. This set of experiments supports, but does not prove, the validity of the proposed method of measuring brain oxygen metabolism.
| Original language | English (US) |
|---|---|
| Article number | A012 |
| Pages (from-to) | 3941-3951 |
| Number of pages | 11 |
| Journal | Biomedical Optics Express |
| Volume | 6 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2015 |
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ASJC Scopus subject areas
- Biotechnology
- Atomic and Molecular Physics, and Optics
Cite this
Cerebral metabolic rate of oxygen (CMRO2) assessed by combined Doppler and spectroscopic OCT. / Chong, Shau Poh; Merkle, Conrad W.; Leahy, Conor; Srinivasan, Vivek.
In: Biomedical Optics Express, Vol. 6, No. 10, A012, 01.10.2015, p. 3941-3951.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cerebral metabolic rate of oxygen (CMRO2) assessed by combined Doppler and spectroscopic OCT
AU - Chong, Shau Poh
AU - Merkle, Conrad W.
AU - Leahy, Conor
AU - Srinivasan, Vivek
PY - 2015/10/1
Y1 - 2015/10/1
N2 - A method of measuring cortical oxygen metabolism in the mouse brain that uses independent quantitative measurements of three key parameters: cerebral blood flow (CBF), arteriovenous oxygen extraction (OE), and hemoglobin concentration ([HbT]) is presented. Measurements were performed using a single visible light spectral/Fourier domain OCT microscope, with Doppler and spectroscopic capabilities, through a thinnedskull cranial window in the mouse brain. Baseline metabolic measurements in mice are shown to be consistent with literature values. Oxygen consumption, as measured by this method, did not change substantially during minor changes either in the fraction of inspired oxygen (FiO2) or in the fraction of inspired carbon dioxide (FiCO2), in spite of larger variations in oxygen saturations. This set of experiments supports, but does not prove, the validity of the proposed method of measuring brain oxygen metabolism.
AB - A method of measuring cortical oxygen metabolism in the mouse brain that uses independent quantitative measurements of three key parameters: cerebral blood flow (CBF), arteriovenous oxygen extraction (OE), and hemoglobin concentration ([HbT]) is presented. Measurements were performed using a single visible light spectral/Fourier domain OCT microscope, with Doppler and spectroscopic capabilities, through a thinnedskull cranial window in the mouse brain. Baseline metabolic measurements in mice are shown to be consistent with literature values. Oxygen consumption, as measured by this method, did not change substantially during minor changes either in the fraction of inspired oxygen (FiO2) or in the fraction of inspired carbon dioxide (FiCO2), in spite of larger variations in oxygen saturations. This set of experiments supports, but does not prove, the validity of the proposed method of measuring brain oxygen metabolism.
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UR - http://www.scopus.com/inward/citedby.url?scp=84948732160&partnerID=8YFLogxK
U2 - 10.1364/BOE.6.003941
DO - 10.1364/BOE.6.003941
M3 - Article
AN - SCOPUS:84948732160
VL - 6
SP - 3941
EP - 3951
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 10
M1 - A012
ER -