Cerebral metabolic rate of oxygen (CMRO2) assessed by combined Doppler and spectroscopic OCT

Shau Poh Chong, Conrad W. Merkle, Conor Leahy, Vivek J. Srinivasan

Research output: Contribution to journalArticlepeer-review

50 Scopus citations


A method of measuring cortical oxygen metabolism in the mouse brain that uses independent quantitative measurements of three key parameters: cerebral blood flow (CBF), arteriovenous oxygen extraction (OE), and hemoglobin concentration ([HbT]) is presented. Measurements were performed using a single visible light spectral/Fourier domain OCT microscope, with Doppler and spectroscopic capabilities, through a thinnedskull cranial window in the mouse brain. Baseline metabolic measurements in mice are shown to be consistent with literature values. Oxygen consumption, as measured by this method, did not change substantially during minor changes either in the fraction of inspired oxygen (FiO2) or in the fraction of inspired carbon dioxide (FiCO2), in spite of larger variations in oxygen saturations. This set of experiments supports, but does not prove, the validity of the proposed method of measuring brain oxygen metabolism.

Original languageEnglish (US)
Article numberA012
Pages (from-to)3941-3951
Number of pages11
JournalBiomedical Optics Express
Issue number10
StatePublished - Oct 1 2015

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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