Centriole structure

Mark Winey, Eileen O'Toole

Research output: Contribution to journalReview article

41 Citations (Scopus)

Abstract

Centrioles are among the largest protein-based structures found in most cell types, measuring approximately 250 nm in diameter and approximately 500 nm long in vertebrate cells. Here, we briefly review ultrastructural observations about centrioles and associated structures. At the core of most centrioles is a microtubule scaffold formed from a radial array of nine triplet microtubules. Beyond the microtubule triplets of the centriole, we discuss the critically important cartwheel structure and the more enigmatic luminal density, both found on the inside of the centriole. Finally, we discuss the connectors between centrioles, and the distal and subdistal appendages outside of the microtubule scaffold that reflect centriole age and impart special functions to the centriole. Most of the work we review has been done with electron microscopy or electron tomography of resin-embedded samples, but we also highlight recent work performed with cryoelectron microscopy, cryotomography and subvolume averaging. Significant opportunities remain in the description of centriolar structure, both in mapping of component proteins within the structure and in determining the effect of mutations on components that contribute to the structure and function of the centriole.

Original languageEnglish (US)
JournalPhilosophical transactions of the Royal Society of London. Series B, Biological sciences
Volume369
Issue number1650
DOIs
StatePublished - Sep 5 2014
Externally publishedYes

Fingerprint

Centrioles
centrioles
Scaffolds
Electron microscopy
Tomography
Microtubules
Microscopic examination
microtubules
Proteins
Resins
Electrons
Electron Microscope Tomography
Cryoelectron Microscopy
tomography
appendages
resins
Vertebrates
electron microscopy
Electron Microscopy
proteins

Keywords

  • cartwheel
  • distal appendages
  • luminal density
  • pericentriolar material
  • subdistal appendages
  • triplet microtubules

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Centriole structure. / Winey, Mark; O'Toole, Eileen.

In: Philosophical transactions of the Royal Society of London. Series B, Biological sciences, Vol. 369, No. 1650, 05.09.2014.

Research output: Contribution to journalReview article

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