Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus

Jianli Shi, Ying Zhao, Tyson Vonderfecht, Mark Winey, Michael W. Klymkowsky

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Centrins (Cetns) are highly conserved, widely expressed, and multifunctional Ca 2+ -binding eukaryotic signature proteins best known for their roles in ciliogenesis and as critical components of the global genome nucleotide excision repair system. Two distinct Cetn subtypes, Cetn2-like and Cetn3-like, have been recognized and implicated in a range of cellular processes. In the course of morpholino-based loss of function studies in Xenopus laevis, we have identified a previously unreported Cetn2-specific function, namely in fibroblast growth factor (FGF) mediated signaling, specifically through the regulation of FGF and FGF receptor RNA levels. Cetn2 was found associated with the RNA polymerase II binding sites of the Cetn2-regulated FGF8 and FGFR1a genes, but not at the promoter of a gene (BMP4) whose expression was altered indirectly in Cent2 morphant embryos. These observations point to a previously unexpected role of Cetn2 in the regulation of gene expression and embryonic development.

Original languageEnglish (US)
Article number10283
JournalScientific Reports
Volume5
DOIs
StatePublished - May 27 2015
Externally publishedYes

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Fibroblast Growth Factors
Sulfamethoxazole Drug Combination Trimethoprim
Xenopus
Gene Expression
Genome Components
Fibroblast Growth Factor Receptors
Morpholinos
RNA Polymerase II
Xenopus laevis
Gene Expression Regulation
DNA Repair
Genes
Embryonic Development
Embryonic Structures
Binding Sites
RNA
Proteins

ASJC Scopus subject areas

  • General

Cite this

Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus. / Shi, Jianli; Zhao, Ying; Vonderfecht, Tyson; Winey, Mark; Klymkowsky, Michael W.

In: Scientific Reports, Vol. 5, 10283, 27.05.2015.

Research output: Contribution to journalArticle

Shi, Jianli ; Zhao, Ying ; Vonderfecht, Tyson ; Winey, Mark ; Klymkowsky, Michael W. / Centrin-2 (Cetn2) mediated regulation of FGF/FGFR gene expression in Xenopus. In: Scientific Reports. 2015 ; Vol. 5.
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