Central Fos expression and conditioned flavor avoidance in rats following intragastric administration of bitter taste receptor ligands

Shuzhen Hao, Michelle Dulake, Elvis Espero, Catia Sternini, Helen E Raybould, Linda Rinaman

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

G protein-coupled receptors that signal bitter taste (T2Rs) are expressed in the mucosal lining of the oral cavity and gastrointestinal (GI) tract. In mice, intragastric infusion of T2R ligands activates Fos expression within the caudal viscerosensory portion of the nucleus of the solitary tract (NTS) through a vagal pathway (Hao S, Sternini C, Raybould HE. Am J Physiol Regul Integr Comp Physiol 294: R33-R38, 2008). The present study was performed in rats to further characterize the distribution and chemical phenotypes of brain stem and forebrain neurons activated to express Fos after intragastric gavage of T2R ligands, and to determine a potential behavioral correlate of this central neural activation. Compared with relatively low brain stem and forebrain Fos expression in control rats gavaged intragastrically with water, rats gavaged intragastrically with T2R ligands displayed significantly increased activation of neurons within the caudal medial (visceral) NTS and caudal ventrolateral medulla, including noradrenergic neurons, and within the lateral parabrachial nucleus, central nucleus of the amygdala, and paraventricular nucleus of the hypothalamus. A behavioral correlate of this Fos activation was evidenced when rats avoided consuming flavors that previously were paired with intragastric gavage of T2R ligands. While unconditioned aversive responses to bitter tastants in the oral cavity are often sufficient to inhibit further consumption, a second line of defense may be provided postingestively by ligand-induced signaling at GI T2Rs that signal the brain via vagal sensory inputs to the caudal medulla.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume296
Issue number3
DOIs
StatePublished - Mar 2009

Fingerprint

Ligands
Prosencephalon
Brain Stem
Mouth
Neurons
Adrenergic Neurons
Solitary Nucleus
Paraventricular Hypothalamic Nucleus
G-Protein-Coupled Receptors
Hypothalamus
Gastrointestinal Tract
Phenotype
Water
Brain

Keywords

  • Brain stem
  • Denatonium
  • Forebrain
  • Nucleus of the solitary tract

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Central Fos expression and conditioned flavor avoidance in rats following intragastric administration of bitter taste receptor ligands. / Hao, Shuzhen; Dulake, Michelle; Espero, Elvis; Sternini, Catia; Raybould, Helen E; Rinaman, Linda.

In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 296, No. 3, 03.2009.

Research output: Contribution to journalArticle

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