Cellular stress in Xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with poly(A)-binding protein

Christopher S. Fraser, Virginia M. Pain, Simon J. Morley

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Eukaryotic initiation factor (eIF) 4E binds to the 5'-cap structure of eukaryotic mRNA and has a central role in the control of cell proliferation. We have shown previously that the stimulation of cultured Xenopus kidney cells with serum resulted in the activation of protein synthesis, enhanced phosphorylation of eIF4E and increased binding of the adapter protein, eIF4G, and poly(A)-binding protein (PABP) to eIF4E to form the functional initiation factor complex, eIF4F/PABP. We now show that cellular stresses such as arsenite, anisomycin and heat shock also result in increased phosphorylation of eIF4E, eIF4F complex formation and the association of PABP with eIF4G, in conditions under which the rate of protein synthesis is severely inhibited. In contrast with reported effects on mammalian cells, the stress-induced increase in eIF4F complex formation occurs in the absence of changes in the association of eIF4E with its binding proteins 4E-BPI or 4E-BP2. The stress-induced changes in eIF4E phosphorylation were totally abrogated by the p38 mitogen-activated protein (MAP) kinase inhibitor SB203580, and were partly inhibited by the phosphoinositide 3-kinase inhibitor LY294002 and the mammalian target of rapamycin (mTOR) inhibitor rapamycin. However, eIF4E phosphorylation was unaffected by extracellular signal-regulated protein kinase (MAP kinase) inhibitor PD98059. These results indicate that cellular stresses activate multiple signalling pathways that converge at the level of eIF4F complex formation to influence the interactions between eIF4E, eIF4G and PABP.

Original languageEnglish (US)
Pages (from-to)519-526
Number of pages8
JournalBiochemical Journal
Volume342
Issue number3
DOIs
StatePublished - Sep 15 1999
Externally publishedYes

Fingerprint

Poly(A)-Binding Proteins
Eukaryotic Initiation Factor-4E
Eukaryotic Initiation Factors
Phosphorylation
Xenopus
Association reactions
Kidney
Sirolimus
Protein Kinase Inhibitors
Carrier Proteins
RNA Caps
Anisomycin
Peptide Initiation Factors
Proteins
2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
1-Phosphatidylinositol 4-Kinase
Extracellular Signal-Regulated MAP Kinases
Cell proliferation
p38 Mitogen-Activated Protein Kinases
Phosphatidylinositols

Keywords

  • eIF2α
  • eIF4F
  • p38 mitogen-activated protein kinase
  • Protein synthesis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cellular stress in Xenopus kidney cells enhances the phosphorylation of eukaryotic translation initiation factor (eIF)4E and the association of eIF4F with poly(A)-binding protein. / Fraser, Christopher S.; Pain, Virginia M.; Morley, Simon J.

In: Biochemical Journal, Vol. 342, No. 3, 15.09.1999, p. 519-526.

Research output: Contribution to journalArticle

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