Cellular receptors to the anaphylatoxins C3a and C5a.

R. Huey, Y. Fukuoka, P. D. Hoeprich, T. E. Hugli

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A single component in the plasma membrane of human polymorphonuclear leukocytes (hPMN) has been identified as the binding site for C5a. Labelled human C5a can be cross-linked to a 48,000-Mr membrane component on the hPMN surface by using the bifunctional reagent ethylene glycol bis(succinimidyl succinate). The membrane component is believed to be the C5a receptor or the binding subunit of a C5a receptor complex. Our ligand-uptake data indicate that the hPMN has high-affinity binding sites for C5a with a Kd of the order of 1-2 nM and an estimated 50,000-113,000 binding sites/cell. Preliminary binding studies of C3a and C5a to rat peritoneal mast cells indicate that non-specific uptake by these cells is so great that it obscures characterization of specific receptor interactions. Data recently reported [Gervasoni, Conrad, Hugli, Schwartz & Ruddy (1986) J. Immunol. 136, 285-292] suggest that non-specific binding of C3a to mast cells is caused by electrostatic interactions between the cationic ligand and anionic heparin-proteoglycan on the cell surface, with an additional complication of the bound ligand undergoing proteolytic degradation. It is therefore proposed that synthetic analogue peptides designed to minimize non-specific interactions with the cell will be useful tools for demonstrating anaphylatoxin receptors on mast cells and may prove essential for receptor isolation.

Original languageEnglish (US)
Pages (from-to)69-81
Number of pages13
JournalBiochemical Society Symposia
Volume51
StatePublished - 1986
Externally publishedYes

Fingerprint

Anaphylatoxin C5a Receptor
Anaphylatoxins
Binding Sites
Mast Cells
Ligands
Neutrophils
Cross-Linking Reagents
Membranes
Cell membranes
Coulomb interactions
Rats
Static Electricity
Cell Communication
Degradation
Peptides
Cell Membrane

ASJC Scopus subject areas

  • Biochemistry

Cite this

Huey, R., Fukuoka, Y., Hoeprich, P. D., & Hugli, T. E. (1986). Cellular receptors to the anaphylatoxins C3a and C5a. Biochemical Society Symposia, 51, 69-81.

Cellular receptors to the anaphylatoxins C3a and C5a. / Huey, R.; Fukuoka, Y.; Hoeprich, P. D.; Hugli, T. E.

In: Biochemical Society Symposia, Vol. 51, 1986, p. 69-81.

Research output: Contribution to journalArticle

Huey, R, Fukuoka, Y, Hoeprich, PD & Hugli, TE 1986, 'Cellular receptors to the anaphylatoxins C3a and C5a.', Biochemical Society Symposia, vol. 51, pp. 69-81.
Huey R, Fukuoka Y, Hoeprich PD, Hugli TE. Cellular receptors to the anaphylatoxins C3a and C5a. Biochemical Society Symposia. 1986;51:69-81.
Huey, R. ; Fukuoka, Y. ; Hoeprich, P. D. ; Hugli, T. E. / Cellular receptors to the anaphylatoxins C3a and C5a. In: Biochemical Society Symposia. 1986 ; Vol. 51. pp. 69-81.
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