Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure

David J. Gordon; Anne E. Milner; Ronald P. Beaney; David J. Grdina; Andrew T M Vaughan

doi:10.1016/0360-3016(90)90228-C

Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure

David J. Gordon, Anne E. Milner, Ronald P. Beaney, David J. Grdina, Andrew T M Vaughan

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

V79 cells grown as spheroids are more radioresistant than those grown as monolayers. Viable cells from spheroid culture contain restraints to ethidium bromide driven rewinding of DNA supercoils that are absent in monlayer cells. Spheroid cells also contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be related to a 55-60 kD protein in the nucleoids of spheroid, but not monolayer cells. Spheroid cell radioresistance may therefore be related to a more stable chromatin platform for high fidelity repair of DNA damage.

Original language	English (US)
Pages (from-to)	1199-1201
Number of pages	3
Journal	International Journal of Radiation Oncology, Biology, Physics
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/0360-3016(90)90228-C
State	Published - 1990
Externally published	Yes

Keywords

Chromatin structure
Radiosensitivity
V79 cells

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Radiation

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10.1016/0360-3016(90)90228-C

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title = "Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure",

abstract = "V79 cells grown as spheroids are more radioresistant than those grown as monolayers. Viable cells from spheroid culture contain restraints to ethidium bromide driven rewinding of DNA supercoils that are absent in monlayer cells. Spheroid cells also contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be related to a 55-60 kD protein in the nucleoids of spheroid, but not monolayer cells. Spheroid cell radioresistance may therefore be related to a more stable chromatin platform for high fidelity repair of DNA damage.",

keywords = "Chromatin structure, Radiosensitivity, V79 cells",

author = "Gordon, {David J.} and Milner, {Anne E.} and Beaney, {Ronald P.} and Grdina, {David J.} and Vaughan, {Andrew T M}",

year = "1990",

doi = "10.1016/0360-3016(90)90228-C",

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pages = "1199--1201",

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T1 - Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure

AU - Gordon, David J.

AU - Milner, Anne E.

AU - Beaney, Ronald P.

AU - Grdina, David J.

AU - Vaughan, Andrew T M

PY - 1990

Y1 - 1990

N2 - V79 cells grown as spheroids are more radioresistant than those grown as monolayers. Viable cells from spheroid culture contain restraints to ethidium bromide driven rewinding of DNA supercoils that are absent in monlayer cells. Spheroid cells also contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be related to a 55-60 kD protein in the nucleoids of spheroid, but not monolayer cells. Spheroid cell radioresistance may therefore be related to a more stable chromatin platform for high fidelity repair of DNA damage.

AB - V79 cells grown as spheroids are more radioresistant than those grown as monolayers. Viable cells from spheroid culture contain restraints to ethidium bromide driven rewinding of DNA supercoils that are absent in monlayer cells. Spheroid cells also contain a DNA-protein matrix that is more resistant to detergent-induced degradation. The increase in structural integrity may be related to a 55-60 kD protein in the nucleoids of spheroid, but not monolayer cells. Spheroid cell radioresistance may therefore be related to a more stable chromatin platform for high fidelity repair of DNA damage.

KW - Chromatin structure

KW - Radiosensitivity

KW - V79 cells

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JF - International Journal of Radiation Oncology Biology Physics

SN - 0360-3016

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Cellular radiosensitivity in V79 cells is linked to alterations in chromatin structure

Abstract

Keywords

ASJC Scopus subject areas

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