Cellular micropatterns with built-in biosensors for detection of extracellular metabolites

J. Yan, J. Y. Lee, S. Shah, A. Revzin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a strategy for integrating cells and biosensors based on nonfouling poly (ethylene glycol) (PEG) hydrogel microstructures serving a dual role of cell culture chambers and matrices for encapsulation of sensing elements. We demonstrated incorporation of biosensors into micropatterned mono and co-cultures, and sucessfully detected release of endogenous hydrogen peroxide (H2O2) by macrophages. This strategy for integrating cells and miniature sensing elements may be used in the future for detection of multiple metabolites from a small group of cells.

Original languageEnglish (US)
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages1849-1851
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Biosensors
  • Cell micropatterning
  • Hydrogel microstructures
  • Microfabrication

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Yan, J., Lee, J. Y., Shah, S., & Revzin, A. (2008). Cellular micropatterns with built-in biosensors for detection of extracellular metabolites. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 1849-1851). Chemical and Biological Microsystems Society.