Abstract
Peripheral cellular immunity of ten dogs with keratoconjunctivitis sicca (KCS) that had not been treated with topical corticosteroids or cyclosporine was evaluated (by use of in vitro lymphocyte proliferation assays and CD4+ CD8+ lymphocyte subset analysis) before and after 1 and 3 months of treatment with topical ocular 2% cyclosporine (CsA). In vitro lymphocyte proliferation and CD4+ CD8+ lymphocyte subset analysis was done in eight normal dogs at the 0, 1 and 3 month time periods to use for comparison. There was no significant difference in lymphocyte proliferation or numbers of CD4+ or CD8+ lymphocytes in dogs with KCS and normal dogs prior to CsA treatment. However, by 1 month's time, lymphocyte proliferation had decreased in the CsA-treated Dogs with KCS, and by 3 months there was a significant difference (P<0.0001) from the normal dogs. These results suggest that dogs with KCS may not have altered peripheral cellular immunity and that use of topical 2% cyclosporine for treatment of KCS causes a suppression of lymphocyte proliferation after 1 to 3 months of use.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 199-208 |
| Number of pages | 10 |
| Journal | Veterinary Immunology and Immunopathology |
| Volume | 49 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
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Keywords
- Canine
- Cellular immunity
- Cyclosporine
- Keratoconjunctivitis sicca
ASJC Scopus subject areas
- Immunology
- veterinary(all)
- Animal Science and Zoology
Cite this
Cellular immunity in dogs with keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine. / Gilger, Brian C.; Andrews, Janice; Wilkie, David A.; Wyman, Milton; Lairmore, Michael Dale.
In: Veterinary Immunology and Immunopathology, Vol. 49, No. 3, 1995, p. 199-208.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular immunity in dogs with keratoconjunctivitis sicca before and after treatment with topical 2% cyclosporine
AU - Gilger, Brian C.
AU - Andrews, Janice
AU - Wilkie, David A.
AU - Wyman, Milton
AU - Lairmore, Michael Dale
PY - 1995
Y1 - 1995
N2 - Peripheral cellular immunity of ten dogs with keratoconjunctivitis sicca (KCS) that had not been treated with topical corticosteroids or cyclosporine was evaluated (by use of in vitro lymphocyte proliferation assays and CD4+ CD8+ lymphocyte subset analysis) before and after 1 and 3 months of treatment with topical ocular 2% cyclosporine (CsA). In vitro lymphocyte proliferation and CD4+ CD8+ lymphocyte subset analysis was done in eight normal dogs at the 0, 1 and 3 month time periods to use for comparison. There was no significant difference in lymphocyte proliferation or numbers of CD4+ or CD8+ lymphocytes in dogs with KCS and normal dogs prior to CsA treatment. However, by 1 month's time, lymphocyte proliferation had decreased in the CsA-treated Dogs with KCS, and by 3 months there was a significant difference (P<0.0001) from the normal dogs. These results suggest that dogs with KCS may not have altered peripheral cellular immunity and that use of topical 2% cyclosporine for treatment of KCS causes a suppression of lymphocyte proliferation after 1 to 3 months of use.
AB - Peripheral cellular immunity of ten dogs with keratoconjunctivitis sicca (KCS) that had not been treated with topical corticosteroids or cyclosporine was evaluated (by use of in vitro lymphocyte proliferation assays and CD4+ CD8+ lymphocyte subset analysis) before and after 1 and 3 months of treatment with topical ocular 2% cyclosporine (CsA). In vitro lymphocyte proliferation and CD4+ CD8+ lymphocyte subset analysis was done in eight normal dogs at the 0, 1 and 3 month time periods to use for comparison. There was no significant difference in lymphocyte proliferation or numbers of CD4+ or CD8+ lymphocytes in dogs with KCS and normal dogs prior to CsA treatment. However, by 1 month's time, lymphocyte proliferation had decreased in the CsA-treated Dogs with KCS, and by 3 months there was a significant difference (P<0.0001) from the normal dogs. These results suggest that dogs with KCS may not have altered peripheral cellular immunity and that use of topical 2% cyclosporine for treatment of KCS causes a suppression of lymphocyte proliferation after 1 to 3 months of use.
KW - Canine
KW - Cellular immunity
KW - Cyclosporine
KW - Keratoconjunctivitis sicca
UR - http://www.scopus.com/inward/record.url?scp=0029608817&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029608817&partnerID=8YFLogxK
U2 - 10.1016/0165-2427(95)05471-5
DO - 10.1016/0165-2427(95)05471-5
M3 - Article
VL - 49
SP - 199
EP - 208
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
SN - 0165-2427
IS - 3
ER -