Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines

Xiaosong He, Tyson H. Holmes, Caiqiu Zhang, Kutubuddin Mahmood, George W. Kemble, David B. Lewis, Cornelia L. Dekker, Harry B. Greenberg, Ann M. Arvin

Research output: Contribution to journalArticle

226 Citations (Scopus)

Abstract

The patterns of cellular immune responses induced by live attenuated influenza vaccine (LAIV) versus those of the trivalent inactivated influenza vaccine (TIV) have not been studied extensively, especially in children. The goals of this study were to evaluate the effects of TIV and LAIV immunization on cellular immunity to live influenza A virus in children and adults and to explore factors associated with variations in responses to influenza vaccines among individuals. A gamma interferon (IFN-γ) flow cytometry assay was used to measure IFN-γ-producing (IFN-γ+) NK and T cells in peripheral blood mononuclear cell cultures stimulated with a live influenza A virus strain before and after LAIV or TIV immunization of children and adults. The mean percentages of influenza A virus-specific IFN-γ+ CD4 and CD8 T cells increased significantly after LAIV, but not TIV, immunization in children aged 5 to 9 years. No increases in the mean levels of influenza A virus-reactive IFN-γ+ T cells and NK cells were observed in adults given LAIV or TIV. TIV induced a significant increase in influenza A virus-reactive T cells in 6-month- to 4-year-old children; LAIV was not evaluated in this age group. The postvaccination changes (n-fold) in the percentages of influenza A virus-reactive IFN-γ+ T and NK cells in adults were highly variable and correlated inversely with the prevaccination percentages, in particular with that of the CD56dim NK cell subset. In conclusion, our findings identify age, type of vaccine, and prevaccination levels of immune reactivity to influenza A virus as factors significantly associated with the magnitude of cellular immune responses to influenza vaccines.

Original languageEnglish (US)
Pages (from-to)11756-11766
Number of pages11
JournalJournal of Virology
Volume80
Issue number23
DOIs
StatePublished - Dec 2006
Externally publishedYes

Fingerprint

Attenuated Vaccines
Influenza Vaccines
Cellular Immunity
influenza
cell-mediated immunity
vaccines
Influenza A virus
Inactivated Vaccines
interferons
Interferons
natural killer cells
Natural Killer Cells
T-lymphocytes
T-Lymphocytes
Immunization
immunization
mononuclear leukocytes
interferon-gamma
Interferon-gamma

ASJC Scopus subject areas

  • Immunology

Cite this

He, X., Holmes, T. H., Zhang, C., Mahmood, K., Kemble, G. W., Lewis, D. B., ... Arvin, A. M. (2006). Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines. Journal of Virology, 80(23), 11756-11766. https://doi.org/10.1128/JVI.01460-06

Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines. / He, Xiaosong; Holmes, Tyson H.; Zhang, Caiqiu; Mahmood, Kutubuddin; Kemble, George W.; Lewis, David B.; Dekker, Cornelia L.; Greenberg, Harry B.; Arvin, Ann M.

In: Journal of Virology, Vol. 80, No. 23, 12.2006, p. 11756-11766.

Research output: Contribution to journalArticle

He, X, Holmes, TH, Zhang, C, Mahmood, K, Kemble, GW, Lewis, DB, Dekker, CL, Greenberg, HB & Arvin, AM 2006, 'Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines', Journal of Virology, vol. 80, no. 23, pp. 11756-11766. https://doi.org/10.1128/JVI.01460-06
He, Xiaosong ; Holmes, Tyson H. ; Zhang, Caiqiu ; Mahmood, Kutubuddin ; Kemble, George W. ; Lewis, David B. ; Dekker, Cornelia L. ; Greenberg, Harry B. ; Arvin, Ann M. / Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines. In: Journal of Virology. 2006 ; Vol. 80, No. 23. pp. 11756-11766.
@article{5529c2fec3874af5afdf7d43066e4e54,
title = "Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines",
abstract = "The patterns of cellular immune responses induced by live attenuated influenza vaccine (LAIV) versus those of the trivalent inactivated influenza vaccine (TIV) have not been studied extensively, especially in children. The goals of this study were to evaluate the effects of TIV and LAIV immunization on cellular immunity to live influenza A virus in children and adults and to explore factors associated with variations in responses to influenza vaccines among individuals. A gamma interferon (IFN-γ) flow cytometry assay was used to measure IFN-γ-producing (IFN-γ+) NK and T cells in peripheral blood mononuclear cell cultures stimulated with a live influenza A virus strain before and after LAIV or TIV immunization of children and adults. The mean percentages of influenza A virus-specific IFN-γ+ CD4 and CD8 T cells increased significantly after LAIV, but not TIV, immunization in children aged 5 to 9 years. No increases in the mean levels of influenza A virus-reactive IFN-γ+ T cells and NK cells were observed in adults given LAIV or TIV. TIV induced a significant increase in influenza A virus-reactive T cells in 6-month- to 4-year-old children; LAIV was not evaluated in this age group. The postvaccination changes (n-fold) in the percentages of influenza A virus-reactive IFN-γ+ T and NK cells in adults were highly variable and correlated inversely with the prevaccination percentages, in particular with that of the CD56dim NK cell subset. In conclusion, our findings identify age, type of vaccine, and prevaccination levels of immune reactivity to influenza A virus as factors significantly associated with the magnitude of cellular immune responses to influenza vaccines.",
author = "Xiaosong He and Holmes, {Tyson H.} and Caiqiu Zhang and Kutubuddin Mahmood and Kemble, {George W.} and Lewis, {David B.} and Dekker, {Cornelia L.} and Greenberg, {Harry B.} and Arvin, {Ann M.}",
year = "2006",
month = "12",
doi = "10.1128/JVI.01460-06",
language = "English (US)",
volume = "80",
pages = "11756--11766",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "23",

}

TY - JOUR

T1 - Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines

AU - He, Xiaosong

AU - Holmes, Tyson H.

AU - Zhang, Caiqiu

AU - Mahmood, Kutubuddin

AU - Kemble, George W.

AU - Lewis, David B.

AU - Dekker, Cornelia L.

AU - Greenberg, Harry B.

AU - Arvin, Ann M.

PY - 2006/12

Y1 - 2006/12

N2 - The patterns of cellular immune responses induced by live attenuated influenza vaccine (LAIV) versus those of the trivalent inactivated influenza vaccine (TIV) have not been studied extensively, especially in children. The goals of this study were to evaluate the effects of TIV and LAIV immunization on cellular immunity to live influenza A virus in children and adults and to explore factors associated with variations in responses to influenza vaccines among individuals. A gamma interferon (IFN-γ) flow cytometry assay was used to measure IFN-γ-producing (IFN-γ+) NK and T cells in peripheral blood mononuclear cell cultures stimulated with a live influenza A virus strain before and after LAIV or TIV immunization of children and adults. The mean percentages of influenza A virus-specific IFN-γ+ CD4 and CD8 T cells increased significantly after LAIV, but not TIV, immunization in children aged 5 to 9 years. No increases in the mean levels of influenza A virus-reactive IFN-γ+ T cells and NK cells were observed in adults given LAIV or TIV. TIV induced a significant increase in influenza A virus-reactive T cells in 6-month- to 4-year-old children; LAIV was not evaluated in this age group. The postvaccination changes (n-fold) in the percentages of influenza A virus-reactive IFN-γ+ T and NK cells in adults were highly variable and correlated inversely with the prevaccination percentages, in particular with that of the CD56dim NK cell subset. In conclusion, our findings identify age, type of vaccine, and prevaccination levels of immune reactivity to influenza A virus as factors significantly associated with the magnitude of cellular immune responses to influenza vaccines.

AB - The patterns of cellular immune responses induced by live attenuated influenza vaccine (LAIV) versus those of the trivalent inactivated influenza vaccine (TIV) have not been studied extensively, especially in children. The goals of this study were to evaluate the effects of TIV and LAIV immunization on cellular immunity to live influenza A virus in children and adults and to explore factors associated with variations in responses to influenza vaccines among individuals. A gamma interferon (IFN-γ) flow cytometry assay was used to measure IFN-γ-producing (IFN-γ+) NK and T cells in peripheral blood mononuclear cell cultures stimulated with a live influenza A virus strain before and after LAIV or TIV immunization of children and adults. The mean percentages of influenza A virus-specific IFN-γ+ CD4 and CD8 T cells increased significantly after LAIV, but not TIV, immunization in children aged 5 to 9 years. No increases in the mean levels of influenza A virus-reactive IFN-γ+ T cells and NK cells were observed in adults given LAIV or TIV. TIV induced a significant increase in influenza A virus-reactive T cells in 6-month- to 4-year-old children; LAIV was not evaluated in this age group. The postvaccination changes (n-fold) in the percentages of influenza A virus-reactive IFN-γ+ T and NK cells in adults were highly variable and correlated inversely with the prevaccination percentages, in particular with that of the CD56dim NK cell subset. In conclusion, our findings identify age, type of vaccine, and prevaccination levels of immune reactivity to influenza A virus as factors significantly associated with the magnitude of cellular immune responses to influenza vaccines.

UR - http://www.scopus.com/inward/record.url?scp=33751251998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751251998&partnerID=8YFLogxK

U2 - 10.1128/JVI.01460-06

DO - 10.1128/JVI.01460-06

M3 - Article

C2 - 16971435

AN - SCOPUS:33751251998

VL - 80

SP - 11756

EP - 11766

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 23

ER -