Cellular factors regulate transactivation of human immunodeficiency virus type 1

Peter A Barry, Elissa Pratt-Lowe, Ronald E. Unger, Paul A Luciw

Research output: Contribution to journalArticle

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Abstract

It is hypothesized that the immediate-early (IE) gene products of human cytomegatovirus (CMV) and the transactivator (TAT) of human immunodeficiency virus type 1 (HIV-1) regulate HIV-1 gene expression through mechanisms involving host cell factors. By using transient transfection assays with the gene for chloramphenicol acetyltransferase (CAT) under the transcriptional control of the HIV-1 long terminal repeat (LTR), we examined transactivation of the LTR by plasmids that express either the HIV-1 gene for TAT or human CMV IE, The ratio of the level of transactivation by CMV IE to the level of transactivation by TAT varied up to 1,000-fold between cell types. The difference in the activities of these transactivators in various cell types was not a consequence of differential expression of the transactivator gene. Analysis of RNA species initiated in the HIV-1 LTR supports the conclusion that cellular factors regulate the level of elongation of the transcription complex on the LTR. Furthermore, evidence that in some cell types the predominant mechanism of transactivation by HIV-1 TAT involves posttranscriptional processes is presented.

Original languageEnglish (US)
Pages (from-to)1392-1399
Number of pages8
JournalJournal of Virology
Volume65
Issue number3
StatePublished - 1991

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Trans-Activators
transcriptional activation
Human immunodeficiency virus 1
Transcriptional Activation
HIV-1
terminal repeat sequences
HIV Long Terminal Repeat
Terminal Repeat Sequences
genes
cells
chloramphenicol acetyltransferase
Gene Expression
Chloramphenicol O-Acetyltransferase
Immediate-Early Genes
transfection
Genes
Transfection
transactivators
plasmids
Plasmids

ASJC Scopus subject areas

  • Immunology

Cite this

Cellular factors regulate transactivation of human immunodeficiency virus type 1. / Barry, Peter A; Pratt-Lowe, Elissa; Unger, Ronald E.; Luciw, Paul A.

In: Journal of Virology, Vol. 65, No. 3, 1991, p. 1392-1399.

Research output: Contribution to journalArticle

Barry, Peter A ; Pratt-Lowe, Elissa ; Unger, Ronald E. ; Luciw, Paul A. / Cellular factors regulate transactivation of human immunodeficiency virus type 1. In: Journal of Virology. 1991 ; Vol. 65, No. 3. pp. 1392-1399.
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