Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR

Chengji Zhou, Sakae Kikuyama, Motoko Shibanuma, Takahiro Hirabayashi, Shigeo Nakajo, Akira Arimura, Seiji Shioda

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide and its specific receptor (the PAC1 receptor) is widely distributed in the rat brain. It has been reported that alternative splicing of the region encoding the third intracellular loop of the PAC1 receptor generates six isoforms which are differentially coupled to signal transduction pathways, but the precise distribution and localization of these splice isoforms in the brain remain to be determined. Using the initial specific primer pairs which correspond to the 'hip' or 'hop' types of receptors for the solution-phase reverse transcription-polymerase chain reaction (RT-PCR), we demonstrated that the major splice variants of the PAC1 receptor in various regions of the rat brain are the short splice isoform 'PAC1-R-s' which does not contain either the 'hip' or 'hop' cassette and the another splice isoform, 'PAC1-R-hop', which contains the 'hop' cassette. With an innovative molecular histochemical technique, in situ RT-PCR, we determined that these two splice isoforms are both intensely expressed in the mitral cells of the olfactory bulb, the Purkinje cells of the cerebellum, the pyramidal cells of the hippocampus and neocortex, and many neurons in the nuclei of hypothalamus and thalamus as well as other regions. The initial mapping of the cell type-specific expression of these two splice variants of the PAC1 receptor provides the basis for a better understanding of the functional significance of the PAC1-R and its ligand PACAP in various brain regions. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)150-158
Number of pages9
JournalMolecular Brain Research
Volume75
Issue number1
DOIs
StatePublished - Jan 10 2000
Externally publishedYes

Fingerprint

Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Humulus
Reverse Transcription
Protein Isoforms
Polymerase Chain Reaction
Brain
Pituitary Adenylate Cyclase-Activating Polypeptide
Hip
Pyramidal Cells
Olfactory Bulb
Purkinje Cells
Neocortex
Alternative Splicing
Neuropeptides
Thalamus
Cerebellum
Hypothalamus
Signal Transduction
Hippocampus

Keywords

  • Alternative splicing
  • G-protein-coupled receptor
  • In situ RT-PCR
  • PAC receptor
  • PACAP
  • Solution-phase RT-PCR

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR. / Zhou, Chengji; Kikuyama, Sakae; Shibanuma, Motoko; Hirabayashi, Takahiro; Nakajo, Shigeo; Arimura, Akira; Shioda, Seiji.

In: Molecular Brain Research, Vol. 75, No. 1, 10.01.2000, p. 150-158.

Research output: Contribution to journalArticle

Zhou, Chengji ; Kikuyama, Sakae ; Shibanuma, Motoko ; Hirabayashi, Takahiro ; Nakajo, Shigeo ; Arimura, Akira ; Shioda, Seiji. / Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR. In: Molecular Brain Research. 2000 ; Vol. 75, No. 1. pp. 150-158.
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