Abstract
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide and its specific receptor (the PAC1 receptor) is widely distributed in the rat brain. It has been reported that alternative splicing of the region encoding the third intracellular loop of the PAC1 receptor generates six isoforms which are differentially coupled to signal transduction pathways, but the precise distribution and localization of these splice isoforms in the brain remain to be determined. Using the initial specific primer pairs which correspond to the 'hip' or 'hop' types of receptors for the solution-phase reverse transcription-polymerase chain reaction (RT-PCR), we demonstrated that the major splice variants of the PAC1 receptor in various regions of the rat brain are the short splice isoform 'PAC1-R-s' which does not contain either the 'hip' or 'hop' cassette and the another splice isoform, 'PAC1-R-hop', which contains the 'hop' cassette. With an innovative molecular histochemical technique, in situ RT-PCR, we determined that these two splice isoforms are both intensely expressed in the mitral cells of the olfactory bulb, the Purkinje cells of the cerebellum, the pyramidal cells of the hippocampus and neocortex, and many neurons in the nuclei of hypothalamus and thalamus as well as other regions. The initial mapping of the cell type-specific expression of these two splice variants of the PAC1 receptor provides the basis for a better understanding of the functional significance of the PAC1-R and its ligand PACAP in various brain regions. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 150-158 |
| Number of pages | 9 |
| Journal | Molecular Brain Research |
| Volume | 75 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 10 2000 |
| Externally published | Yes |
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Keywords
- Alternative splicing
- G-protein-coupled receptor
- In situ RT-PCR
- PAC receptor
- PACAP
- Solution-phase RT-PCR
ASJC Scopus subject areas
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR. / Zhou, Chengji; Kikuyama, Sakae; Shibanuma, Motoko; Hirabayashi, Takahiro; Nakajo, Shigeo; Arimura, Akira; Shioda, Seiji.
In: Molecular Brain Research, Vol. 75, No. 1, 10.01.2000, p. 150-158.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cellular distribution of the splice variants of the receptor for pituitary adenylate cyclase-activating polypeptide (PAC1-R) in the rat brain by in situ RT-PCR
AU - Zhou, Chengji
AU - Kikuyama, Sakae
AU - Shibanuma, Motoko
AU - Hirabayashi, Takahiro
AU - Nakajo, Shigeo
AU - Arimura, Akira
AU - Shioda, Seiji
PY - 2000/1/10
Y1 - 2000/1/10
N2 - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide and its specific receptor (the PAC1 receptor) is widely distributed in the rat brain. It has been reported that alternative splicing of the region encoding the third intracellular loop of the PAC1 receptor generates six isoforms which are differentially coupled to signal transduction pathways, but the precise distribution and localization of these splice isoforms in the brain remain to be determined. Using the initial specific primer pairs which correspond to the 'hip' or 'hop' types of receptors for the solution-phase reverse transcription-polymerase chain reaction (RT-PCR), we demonstrated that the major splice variants of the PAC1 receptor in various regions of the rat brain are the short splice isoform 'PAC1-R-s' which does not contain either the 'hip' or 'hop' cassette and the another splice isoform, 'PAC1-R-hop', which contains the 'hop' cassette. With an innovative molecular histochemical technique, in situ RT-PCR, we determined that these two splice isoforms are both intensely expressed in the mitral cells of the olfactory bulb, the Purkinje cells of the cerebellum, the pyramidal cells of the hippocampus and neocortex, and many neurons in the nuclei of hypothalamus and thalamus as well as other regions. The initial mapping of the cell type-specific expression of these two splice variants of the PAC1 receptor provides the basis for a better understanding of the functional significance of the PAC1-R and its ligand PACAP in various brain regions. Copyright (C) 2000 Elsevier Science B.V.
AB - Pituitary adenylate cyclase-activating polypeptide (PACAP) is a pleiotropic neuropeptide and its specific receptor (the PAC1 receptor) is widely distributed in the rat brain. It has been reported that alternative splicing of the region encoding the third intracellular loop of the PAC1 receptor generates six isoforms which are differentially coupled to signal transduction pathways, but the precise distribution and localization of these splice isoforms in the brain remain to be determined. Using the initial specific primer pairs which correspond to the 'hip' or 'hop' types of receptors for the solution-phase reverse transcription-polymerase chain reaction (RT-PCR), we demonstrated that the major splice variants of the PAC1 receptor in various regions of the rat brain are the short splice isoform 'PAC1-R-s' which does not contain either the 'hip' or 'hop' cassette and the another splice isoform, 'PAC1-R-hop', which contains the 'hop' cassette. With an innovative molecular histochemical technique, in situ RT-PCR, we determined that these two splice isoforms are both intensely expressed in the mitral cells of the olfactory bulb, the Purkinje cells of the cerebellum, the pyramidal cells of the hippocampus and neocortex, and many neurons in the nuclei of hypothalamus and thalamus as well as other regions. The initial mapping of the cell type-specific expression of these two splice variants of the PAC1 receptor provides the basis for a better understanding of the functional significance of the PAC1-R and its ligand PACAP in various brain regions. Copyright (C) 2000 Elsevier Science B.V.
KW - Alternative splicing
KW - G-protein-coupled receptor
KW - In situ RT-PCR
KW - PAC receptor
KW - PACAP
KW - Solution-phase RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=0033983249&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033983249&partnerID=8YFLogxK
U2 - 10.1016/S0169-328X(99)00300-9
DO - 10.1016/S0169-328X(99)00300-9
M3 - Article
C2 - 10648899
AN - SCOPUS:0033983249
VL - 75
SP - 150
EP - 158
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 1
ER -