Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus

Tomohiro Asai, Mei Ho Lee, Carlos Arrecubieta, Manuel Prinz von Bayern, Christian A. Cespedes, Helen M. Baron, Martin Cadeiras, Taichi Sakaguchi, Charles C. Marboe, Yoshifumi Naka, Mario C. Deng, Franklin D. Lowy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Objective: Infections are among the most common and serious complications of ventricular assist device implantation. These infections generally occur within the first 2 months after surgery. The basis for this high incidence of infection is not well established, so a murine intravascular infection model was developed with aortic implantation of the textured polyurethane patch material currently used in HeartMate ventricular assist devices (Thoratec Corporation Pleasanton, Calif). Methods: Polyurethane patch material was placed in the wall of the mouse descending aorta. Mice were then infected with Staphylococcus aureus 1 or 14 days after implantation. In vitro adhesion studies were conducted with polyurethane membranes coated with endothelial cells and membranes coated with fibrinogen. Results: Mice were susceptible to infection in both dose- and time-dependent fashions. The patch material was significantly more susceptible to infection at day 1 than day 14. Immunohistologic and morphologic studies demonstrated that the CD31+ cells deposited on the membrane surface phenotypically appeared to be endothelial cells. In vitro adhesion studies of polyurethane membranes coated with endothelial cells showed them to be less susceptible to S aureus binding than were membranes coated with fibrinogen. Conclusion: Textured polyurethane membranes are less susceptible to infection as cellular deposition occurs. The time frame within which these membranes become populated with cellular material is consistent with the time-dependent clinical incidence of infection. Cellular coating of polyurethane may provide a strategy for reducing the risk of infection.

Original languageEnglish (US)
Pages (from-to)1147-1153
Number of pages7
JournalJournal of Thoracic and Cardiovascular Surgery
Volume133
Issue number5
DOIs
StatePublished - May 1 2007
Externally publishedYes

Fingerprint

Heart-Assist Devices
Polyurethanes
Staphylococcus aureus
Membranes
Infection
Endothelial Cells
Fibrinogen
Incidence
Thoracic Aorta
Cell Membrane

ASJC Scopus subject areas

  • Surgery
  • Pulmonary and Respiratory Medicine
  • Cardiology and Cardiovascular Medicine

Cite this

Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus. / Asai, Tomohiro; Lee, Mei Ho; Arrecubieta, Carlos; von Bayern, Manuel Prinz; Cespedes, Christian A.; Baron, Helen M.; Cadeiras, Martin; Sakaguchi, Taichi; Marboe, Charles C.; Naka, Yoshifumi; Deng, Mario C.; Lowy, Franklin D.

In: Journal of Thoracic and Cardiovascular Surgery, Vol. 133, No. 5, 01.05.2007, p. 1147-1153.

Research output: Contribution to journalArticle

Asai, T, Lee, MH, Arrecubieta, C, von Bayern, MP, Cespedes, CA, Baron, HM, Cadeiras, M, Sakaguchi, T, Marboe, CC, Naka, Y, Deng, MC & Lowy, FD 2007, 'Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus', Journal of Thoracic and Cardiovascular Surgery, vol. 133, no. 5, pp. 1147-1153. https://doi.org/10.1016/j.jtcvs.2006.10.084
Asai, Tomohiro ; Lee, Mei Ho ; Arrecubieta, Carlos ; von Bayern, Manuel Prinz ; Cespedes, Christian A. ; Baron, Helen M. ; Cadeiras, Martin ; Sakaguchi, Taichi ; Marboe, Charles C. ; Naka, Yoshifumi ; Deng, Mario C. ; Lowy, Franklin D. / Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus. In: Journal of Thoracic and Cardiovascular Surgery. 2007 ; Vol. 133, No. 5. pp. 1147-1153.
@article{5cc0f5d187294011b74df716b3128bd0,
title = "Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus",
abstract = "Objective: Infections are among the most common and serious complications of ventricular assist device implantation. These infections generally occur within the first 2 months after surgery. The basis for this high incidence of infection is not well established, so a murine intravascular infection model was developed with aortic implantation of the textured polyurethane patch material currently used in HeartMate ventricular assist devices (Thoratec Corporation Pleasanton, Calif). Methods: Polyurethane patch material was placed in the wall of the mouse descending aorta. Mice were then infected with Staphylococcus aureus 1 or 14 days after implantation. In vitro adhesion studies were conducted with polyurethane membranes coated with endothelial cells and membranes coated with fibrinogen. Results: Mice were susceptible to infection in both dose- and time-dependent fashions. The patch material was significantly more susceptible to infection at day 1 than day 14. Immunohistologic and morphologic studies demonstrated that the CD31+ cells deposited on the membrane surface phenotypically appeared to be endothelial cells. In vitro adhesion studies of polyurethane membranes coated with endothelial cells showed them to be less susceptible to S aureus binding than were membranes coated with fibrinogen. Conclusion: Textured polyurethane membranes are less susceptible to infection as cellular deposition occurs. The time frame within which these membranes become populated with cellular material is consistent with the time-dependent clinical incidence of infection. Cellular coating of polyurethane may provide a strategy for reducing the risk of infection.",
author = "Tomohiro Asai and Lee, {Mei Ho} and Carlos Arrecubieta and {von Bayern}, {Manuel Prinz} and Cespedes, {Christian A.} and Baron, {Helen M.} and Martin Cadeiras and Taichi Sakaguchi and Marboe, {Charles C.} and Yoshifumi Naka and Deng, {Mario C.} and Lowy, {Franklin D.}",
year = "2007",
month = "5",
day = "1",
doi = "10.1016/j.jtcvs.2006.10.084",
language = "English (US)",
volume = "133",
pages = "1147--1153",
journal = "Journal of Thoracic and Cardiovascular Surgery",
issn = "0022-5223",
publisher = "Mosby Inc.",
number = "5",

}

TY - JOUR

T1 - Cellular coating of the left ventricular assist device textured polyurethane membrane reduces adhesion of Staphylococcus aureus

AU - Asai, Tomohiro

AU - Lee, Mei Ho

AU - Arrecubieta, Carlos

AU - von Bayern, Manuel Prinz

AU - Cespedes, Christian A.

AU - Baron, Helen M.

AU - Cadeiras, Martin

AU - Sakaguchi, Taichi

AU - Marboe, Charles C.

AU - Naka, Yoshifumi

AU - Deng, Mario C.

AU - Lowy, Franklin D.

PY - 2007/5/1

Y1 - 2007/5/1

N2 - Objective: Infections are among the most common and serious complications of ventricular assist device implantation. These infections generally occur within the first 2 months after surgery. The basis for this high incidence of infection is not well established, so a murine intravascular infection model was developed with aortic implantation of the textured polyurethane patch material currently used in HeartMate ventricular assist devices (Thoratec Corporation Pleasanton, Calif). Methods: Polyurethane patch material was placed in the wall of the mouse descending aorta. Mice were then infected with Staphylococcus aureus 1 or 14 days after implantation. In vitro adhesion studies were conducted with polyurethane membranes coated with endothelial cells and membranes coated with fibrinogen. Results: Mice were susceptible to infection in both dose- and time-dependent fashions. The patch material was significantly more susceptible to infection at day 1 than day 14. Immunohistologic and morphologic studies demonstrated that the CD31+ cells deposited on the membrane surface phenotypically appeared to be endothelial cells. In vitro adhesion studies of polyurethane membranes coated with endothelial cells showed them to be less susceptible to S aureus binding than were membranes coated with fibrinogen. Conclusion: Textured polyurethane membranes are less susceptible to infection as cellular deposition occurs. The time frame within which these membranes become populated with cellular material is consistent with the time-dependent clinical incidence of infection. Cellular coating of polyurethane may provide a strategy for reducing the risk of infection.

AB - Objective: Infections are among the most common and serious complications of ventricular assist device implantation. These infections generally occur within the first 2 months after surgery. The basis for this high incidence of infection is not well established, so a murine intravascular infection model was developed with aortic implantation of the textured polyurethane patch material currently used in HeartMate ventricular assist devices (Thoratec Corporation Pleasanton, Calif). Methods: Polyurethane patch material was placed in the wall of the mouse descending aorta. Mice were then infected with Staphylococcus aureus 1 or 14 days after implantation. In vitro adhesion studies were conducted with polyurethane membranes coated with endothelial cells and membranes coated with fibrinogen. Results: Mice were susceptible to infection in both dose- and time-dependent fashions. The patch material was significantly more susceptible to infection at day 1 than day 14. Immunohistologic and morphologic studies demonstrated that the CD31+ cells deposited on the membrane surface phenotypically appeared to be endothelial cells. In vitro adhesion studies of polyurethane membranes coated with endothelial cells showed them to be less susceptible to S aureus binding than were membranes coated with fibrinogen. Conclusion: Textured polyurethane membranes are less susceptible to infection as cellular deposition occurs. The time frame within which these membranes become populated with cellular material is consistent with the time-dependent clinical incidence of infection. Cellular coating of polyurethane may provide a strategy for reducing the risk of infection.

UR - http://www.scopus.com/inward/record.url?scp=34247473850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34247473850&partnerID=8YFLogxK

U2 - 10.1016/j.jtcvs.2006.10.084

DO - 10.1016/j.jtcvs.2006.10.084

M3 - Article

C2 - 17467422

AN - SCOPUS:34247473850

VL - 133

SP - 1147

EP - 1153

JO - Journal of Thoracic and Cardiovascular Surgery

JF - Journal of Thoracic and Cardiovascular Surgery

SN - 0022-5223

IS - 5

ER -