Cellular characterization of leukotoxin diol-induced mitochondrial dysfunction

Marlene F. Sisemore, Jiang Zheng, Joy C. Yang, David A. Thompson, Charles Plopper, Gino A Cortopassi, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


Leukotoxin, a cytochrome P450-derived epoxide of linoleic acid, has been implicated as a causative factor in acute respiratory distress syndrome. Conversion of this fatty acid epoxide to leukotoxin diol by epoxide hydrolase has been hypothesized as the critical activation step in leukotoxin-induced cellular toxicity. In both human and insect cells, we observed that leukotoxin diol causes acute cellular toxicity and that cyclosporin A, an inhibitor of the mitochondrial permeability transition, ameliorates leukotoxin diol-associated toxicity. To evaluate mitochondria as a target of leukotoxin diol, multiple aspects of mitochondrial integrity were evaluated in both cell- and organelle-based assays. Leukotoxin diol specifically activated the mitochondrial permeability transition, resulting in release of cytochrome c and subsequent cell death. Pretreatment with cyclosporin A inhibited these effects and, furthermore, limited in vivo toxicity. While the mechanisms underlying leukotoxin-mediated toxicity remain to be fully elucidated, the observation that leukotoxin diol disrupts mitochondrial function specifically through activation of the mitochondrial permeability transition suggests at least one mechanism through which leukotoxin diol may exert its activity in physiological contexts.

Original languageEnglish (US)
Pages (from-to)32-37
Number of pages6
JournalArchives of Biochemistry and Biophysics
Issue number1
StatePublished - Aug 1 2001


  • Ards
  • Cyclosporin
  • Epome
  • Epoxide
  • Epoxyoctadecamonoenoic
  • Hydrolase
  • Leukotoxin
  • Mitochondria

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


Dive into the research topics of 'Cellular characterization of leukotoxin diol-induced mitochondrial dysfunction'. Together they form a unique fingerprint.

Cite this