Cellular and molecular mechanisms of anti-inflammatory effect of Aflapin: A novel Boswellia serrata extract

Krishanu Sengupta, Jayaprakash N. Kolla, Alluri V. Krishnaraju, Nandini Yalamanchili, Chirravuri V. Rao, Trimurtulu Golakoti, Smriti Raychaudhuri, Siba P Raychaudhuri

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

There is significant number of evidences suggesting the anti-inflammatory properties of gum resin extracts of Boswellia serrata containing 3-O-acetyl-11-keto-β-boswellic acid (AKBA) and their promising potential as therapeutic interventions against inflammatory diseases such as osteoarthritis (OA). Unfortunately, the poor bioavailability of AKBA following oral administration might limit the anti-inflammatory efficacy of standardized Boswellia extract(s). To address this issue, we describe a novel composition called Aflapin, which contains B. serrata extract enriched in AKBA and non-volatile oil portion of B. serrata gum resin. Our observations show that the availability of AKBA in systemic circulation of experimental animals is increased by 51.78% in Aflapin-supplemented animals, in comparison with that of 30% AKBA standardized extract or BE-30 (5-Loxin®). Consistently, Aflapin confers better anti-inflammatory efficacy in Freund's Complete Adjuvant (FCA)-induced inflammation model of Sprague-Dawley rats. Interestingly, in comparison with BE-30, Aflapin® also provides significantly better protection from IL-1β-induced death of human primary chondrocytes and improves glycosaminoglycans production in human chondrocytes. In Tumor necrosis factor alpha (TNFα)-induced human synovial cells, the inhibitory potential of Aflapin (IC50 44.736 ng/ml) on matrix metalloproteinase-3 (MMP-3) production is 14.83% better than that of BE-30 (IC50 52.528 ng/ml). In summary, our observations collectively suggest that both the Boswellia products, BE-30 (5-Loxin®) and Aflapin, exhibit powerful anti-inflammatory efficacy and anti-arthritic potential. In particular, in comparison with BE-30, Aflapin provides more potential benefits in recovering articular cartilage damage or protection from proteolytic degradation due to inflammatory insult in arthritis such as osteoarthritis or rheumatoid arthritis.

Original languageEnglish (US)
Pages (from-to)189-197
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume354
Issue number1-2
DOIs
StatePublished - Aug 2011

Keywords

  • 3-O-acetyl-11-keto-β-boswellic acid
  • Aflapin
  • Boswellia serrata
  • Chondrocytes
  • Matrix metalloproteinase-3
  • Osteoarthritis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

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    Sengupta, K., Kolla, J. N., Krishnaraju, A. V., Yalamanchili, N., Rao, C. V., Golakoti, T., Raychaudhuri, S., & Raychaudhuri, S. P. (2011). Cellular and molecular mechanisms of anti-inflammatory effect of Aflapin: A novel Boswellia serrata extract. Molecular and Cellular Biochemistry, 354(1-2), 189-197. https://doi.org/10.1007/s11010-011-0818-1