Cell type and distribution in the porcine temporomandibular joint disc

Michael S. Detamore, Jay N. Hegde, Rohan R. Wagle, Alejandro J. Almarza, Dina Montufar-Solis, P. Jackie Duke, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Purpose: Surprisingly little is known about the cellular composition of the temporomandibular joint (TMJ) disc, which is a crucial piece of the puzzle in tissue engineering efforts. Toward this end, cell types were identified and quantified regionally in the TMJ disc. Materials and Methods: Porcine TMJ discs were examined by histology, electron microscopy, and immunohistochemistry. Histology consisted of hematoxylin and eosin staining to identify regional variation of cell type and cell numbers. Transmission electron microscopy was used to elucidate differences in organelle content and pericellular matrix between TMJ disc cells and chondrocytes from hyaline cartilage. Immunohistochemistry was used to assess the presence of smooth and skeletal muscle character in the TMJ disc. Results: The overall ratio of fibroblasts to chondrocyte-like cells in the TMJ disc was approximately 2.35 to 1, with the highest relative number of chondrocyte-like cells in the intermediate zone. Electron microscopy revealed distinct differences between TMJ disc chondrocyte-like cells and chondrocytes from hyaline cartilage with respect to organelles and the pericellular region. Immunostaining identified smooth muscle in the form of vessels, which were most prominent in the anterior band. Skeletal muscle was not observed. Conclusion: The cells of the TMJ disc are distinctly different from cells of hyaline cartilage, and consequently should not be referred to as chondrocytes. TMJ disc cells are comprised of heterogeneously distributed subpopulations, with fibroblasts predominating over fibrochondrocytes.

Original languageEnglish (US)
Pages (from-to)243-248
Number of pages6
JournalJournal of Oral and Maxillofacial Surgery
Volume64
Issue number2
DOIs
StatePublished - Feb 2006
Externally publishedYes

ASJC Scopus subject areas

  • Dentistry(all)
  • Surgery

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    Detamore, M. S., Hegde, J. N., Wagle, R. R., Almarza, A. J., Montufar-Solis, D., Duke, P. J., & Athanasiou, K. A. (2006). Cell type and distribution in the porcine temporomandibular joint disc. Journal of Oral and Maxillofacial Surgery, 64(2), 243-248. https://doi.org/10.1016/j.joms.2005.10.009