Cell-specific expression of type II calcium/calmodulin-dependent protein kinase isoforms and glutamate receptors in normal and visually deprived lateral geniculate nucleus of monkeys

Brahim Tighilet, Molly M. Huntsman, Tsutomu Hashikawa, Karl D Murray, Paul J. Isackson, Edward G. Jones

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

In situ hybridization histochemistry and immunocytochemistry were used to map distributions of cells expressing mRNAs encoding α, β, γ, and δ isoforms of type II calcium/calmodulin-dependent protein kinase (CaMKII), α- amino-3-hydroxy-5-methyl-4-isoxazoleproprionate (AMPA)/kainate receptor subunits, (GluR1-7), and N-methyl-D-aspartate (NMDA) receptor subunits, NR1 and NR2A-D, or stained by subunit-specific immunocytochemistry in the dorsal lateral geniculate nuclei of macaque monkeys. Relationships of specific isoforms with particular glutamate receptor types may be important elements in neural plasticity. CaMKII-α is expressed only by neurons in the S laminae and interlaminar plexuses of the dorsal lateral geniculate nucleus, but may form part of a more widely distributed matrix of similar cells extending from the geniculate into adjacent nuclei. CaMKII-β, -γ, and -δ isoforms are expressed by all neurons in principal and S laminae and interlaminar plexuses. In principal laminae, they are down-regulated by monocular deprivation lasting 8-21 days. All glutamate receptor subunits are expressed by neurons in principal and S laminae and interlaminar plexuses. The AMPA/kainate subunits, GluR1, 2, 5, and 7, are expressed at low levels, although GluR1 immunostaining appears selectively to stain interneurons. GluR3 is expressed at weak, GluR 6 at moderate and GluR 4 at high levels. NMDA subunits, NR1 and NR2A, B, and D, are expressed at moderate to low levels. GluR4, GluR6 and NMDA subunits are down-regulated by visual deprivation. CaMKII-α expression is unique in comparison with other CaMKII isoforms which may, therefore, have more generalized roles in cell function. The results demonstrate that all of the isoforms are associated with NMDA receptors and with AMPA receptors enriched with GluR4 subunits, which implies high calcium permeability and rapid gating.

Original languageEnglish (US)
Pages (from-to)278-296
Number of pages19
JournalJournal of Comparative Neurology
Volume390
Issue number2
DOIs
StatePublished - Jan 12 1998
Externally publishedYes

Fingerprint

Geniculate Bodies
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Glutamate Receptors
Haplorhini
Protein Isoforms
AMPA Receptors
N-Methylaspartate
N-Methyl-D-Aspartate Receptors
Neurons
Immunohistochemistry
RNA Isoforms
Kainic Acid Receptors
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Neuronal Plasticity
Kainic Acid
Macaca
Interneurons
In Situ Hybridization
Permeability

Keywords

  • α, β, γ, and δ isoforms
  • Gene expression
  • Immunocytochemistry
  • In situ hybridization
  • NMDA and non-NMDA receptors

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cell-specific expression of type II calcium/calmodulin-dependent protein kinase isoforms and glutamate receptors in normal and visually deprived lateral geniculate nucleus of monkeys. / Tighilet, Brahim; Huntsman, Molly M.; Hashikawa, Tsutomu; Murray, Karl D; Isackson, Paul J.; Jones, Edward G.

In: Journal of Comparative Neurology, Vol. 390, No. 2, 12.01.1998, p. 278-296.

Research output: Contribution to journalArticle

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