Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: Adipogenic and osteogenic capacity

Martin A. Vidal, Gail E. Kilroy, Jill R. Johnson, Mandi J. Lopez, Rustin M. Moore, Jeffrey M. Gimble

Research output: Contribution to journalArticle

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Abstract

Objectives - To characterize equine bone marrow (BM)-derived mesenchymal stem cell (MSC) growth characteristics and frequency as well as their adipogenic and osteogenic differentiation potential. Study Design - In vitro experimental study. Animals - Foals (n=3, age range, 17-51 days) and young horses (n=5, age range, 9 months to 5 years). Methods - Equine MSCs were harvested and isolated from sternal BM aspirates and grown up to passage 10 to determine cell-doubling (CD) characteristics. Limit dilution assays were performed on primary and passaged MSCs to determine the frequency of colony-forming units with a fibroblastic phenotype (CFU-F), and the frequency of MSC differentiation into adipocytes (CFU-Ad) and osteoblasts (CFU-Ob). Results - Initial MSC isolates had a lag phase with a significantly longer CD time (DT=4.9±1.6 days) compared with the average DT (1.4±0.22 days) of subsequent MSC passages. Approximately 1 in 4224±3265 of the total nucleated BM cells displayed fibroblast colony-forming activity. Primary MSCs differentiated in response to adipogenic and osteogenic inductive conditions and maintained their differentiation potential during subsequent passages. Conclusions - The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of foals and young adult horses are similar to those documented for BM MSCs of other mammalian species. Clinical Relevance - The results have direct relevance to the use of BM as a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.

Original languageEnglish (US)
Pages (from-to)601-610
Number of pages10
JournalVeterinary Surgery
Volume35
Issue number7
DOIs
StatePublished - Oct 2006
Externally publishedYes

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stromal cells
Mesenchymal Stromal Cells
bone marrow
Horses
cell growth
Bone Marrow
stem cells
horses
Growth
foals
cell engineering
Cell Engineering
tissue engineering
Adult Stem Cells
Veterinary Medicine
osteoblasts
bone marrow cells
Tissue Engineering
Osteoblasts
adipocytes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells : Adipogenic and osteogenic capacity. / Vidal, Martin A.; Kilroy, Gail E.; Johnson, Jill R.; Lopez, Mandi J.; Moore, Rustin M.; Gimble, Jeffrey M.

In: Veterinary Surgery, Vol. 35, No. 7, 10.2006, p. 601-610.

Research output: Contribution to journalArticle

Vidal, Martin A. ; Kilroy, Gail E. ; Johnson, Jill R. ; Lopez, Mandi J. ; Moore, Rustin M. ; Gimble, Jeffrey M. / Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells : Adipogenic and osteogenic capacity. In: Veterinary Surgery. 2006 ; Vol. 35, No. 7. pp. 601-610.
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