Abstract
The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.
Original language | English (US) |
---|---|
Pages (from-to) | 67-74 |
Number of pages | 8 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 105 |
Issue number | 1 |
DOIs | |
State | Published - Mar 15 1982 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology