Cell-free translation and partial characterization of proenkephalin messenger RNA from bovine striatum

Satya Dandekar; Steven L. Sabol

doi:10.1016/S0006-291X(82)80011-9

Cell-free translation and partial characterization of proenkephalin messenger RNA from bovine striatum

Satya Dandekar, Steven L. Sabol

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. ³⁵S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [³⁵S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be M_r 31,000 ± 1000. Digestion of cell-free translated ³H-labeled proteins of M_r 31,000 ± 3000 yielded both [³H]Metenkephalin and [³H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.

Original language	English (US)
Pages (from-to)	67-74
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	105
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(82)80011-9
State	Published - Mar 15 1982
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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title = "Cell-free translation and partial characterization of proenkephalin messenger RNA from bovine striatum",

abstract = "The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.",

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AU - Dandekar, Satya

AU - Sabol, Steven L.

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N2 - The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.

AB - The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.

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