Cell-free translation and partial characterization of proenkephalin messenger RNA from bovine striatum

Satya Dandekar, Steven L. Sabol

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.

Original languageEnglish (US)
Pages (from-to)67-74
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume105
Issue number1
DOIs
StatePublished - Mar 15 1982
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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