The biosynthesis of the primary precursor of the enkephalins (proenkephalin) was investigated by cell-free translation of bovine striatum polyadenylated RNA. 35S-Labeled proteins encoded by striatum mRNA were digested with trypsin and carboxypeptidase B, and released [35S]Met-enkephalin was identified by immunoprecipitation and high-performance liquid chromatography. The apparent molecular weight of putative proenkephalin synthesized in cell-free systems was found to be Mr 31,000 ± 1000. Digestion of cell-free translated 3H-labeled proteins of Mr 31,000 ± 3000 yielded both [3H]Metenkephalin and [3H]Leu-enkephalin. Putative proenkephalin mRNA of striatum was found to be 1450 ± 200 nucleotides in length.
|Original language||English (US)|
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 15 1982|
ASJC Scopus subject areas
- Molecular Biology