Cell-free expression of functional receptor tyrosine kinases

Wei He, Tiffany M. Scharadin, Matthew Saldana, Candice Gellner, Steven Hoang-Phou, Christina Takanishi, Gregory L. Hura, John A. Tainer, Kermit L Carraway, Paul Henderson, Matthew A Coleman

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.

Original languageEnglish (US)
Article number12896
JournalScientific Reports
Volume5
DOIs
StatePublished - Aug 14 2015

ASJC Scopus subject areas

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    He, W., Scharadin, T. M., Saldana, M., Gellner, C., Hoang-Phou, S., Takanishi, C., Hura, G. L., Tainer, J. A., Carraway, K. L., Henderson, P., & Coleman, M. A. (2015). Cell-free expression of functional receptor tyrosine kinases. Scientific Reports, 5, [12896]. https://doi.org/10.1038/srep12896