Cell-free expression of functional receptor tyrosine kinases

Wei He, Tiffany M. Scharadin, Matthew Saldana, Candice Gellner, Steven Hoang-Phou, Christina Takanishi, Gregory L. Hura, John A. Tainer, Kermit L Carraway, Paul Henderson, Matthew A Coleman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Receptor tyrosine kinases (RTKs) play critical roles in physiological and pathological processes, and are important anticancer drug targets. In vitro mechanistic and drug discovery studies of full-length RTKs require protein that is both fully functional and free from contaminating proteins. Here we describe a rapid cell-free and detergent-free co-translation method for producing full-length and functional ERBB2 and EGFR receptor tyrosine kinases supported by water-soluble apolipoprotein A-I based nanolipoprotein particles.

Original languageEnglish (US)
Article number12896
JournalScientific Reports
Volume5
DOIs
StatePublished - Aug 14 2015

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Receptor Protein-Tyrosine Kinases
Physiological Phenomena
Apolipoprotein A-I
Pathologic Processes
Drug Discovery
Detergents
Water
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • General

Cite this

He, W., Scharadin, T. M., Saldana, M., Gellner, C., Hoang-Phou, S., Takanishi, C., ... Coleman, M. A. (2015). Cell-free expression of functional receptor tyrosine kinases. Scientific Reports, 5, [12896]. https://doi.org/10.1038/srep12896

Cell-free expression of functional receptor tyrosine kinases. / He, Wei; Scharadin, Tiffany M.; Saldana, Matthew; Gellner, Candice; Hoang-Phou, Steven; Takanishi, Christina; Hura, Gregory L.; Tainer, John A.; Carraway, Kermit L; Henderson, Paul; Coleman, Matthew A.

In: Scientific Reports, Vol. 5, 12896, 14.08.2015.

Research output: Contribution to journalArticle

He, W, Scharadin, TM, Saldana, M, Gellner, C, Hoang-Phou, S, Takanishi, C, Hura, GL, Tainer, JA, Carraway, KL, Henderson, P & Coleman, MA 2015, 'Cell-free expression of functional receptor tyrosine kinases', Scientific Reports, vol. 5, 12896. https://doi.org/10.1038/srep12896
He W, Scharadin TM, Saldana M, Gellner C, Hoang-Phou S, Takanishi C et al. Cell-free expression of functional receptor tyrosine kinases. Scientific Reports. 2015 Aug 14;5. 12896. https://doi.org/10.1038/srep12896
He, Wei ; Scharadin, Tiffany M. ; Saldana, Matthew ; Gellner, Candice ; Hoang-Phou, Steven ; Takanishi, Christina ; Hura, Gregory L. ; Tainer, John A. ; Carraway, Kermit L ; Henderson, Paul ; Coleman, Matthew A. / Cell-free expression of functional receptor tyrosine kinases. In: Scientific Reports. 2015 ; Vol. 5.
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