Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A

Maria Mudryj, Stephen H. Devoto, Scott W. Hiebert, Tony Hunter, Jonathon Pines, Joseph R. Nevins

Research output: Contribution to journalArticle

211 Scopus citations

Abstract

We have examined E2F binding activity in extracts of synchronizedNIH 3T3 cells. During the G0 to G1 transition, there is a marked increase in the level of active E2F. Subsequently, there are changes in the nature of E2F-containing complexes. A G1-specific complex increases in abundance, disappears, and is then replaced by another complex as S phase begins. Analysis of extracts of thymidine-blocked cells confirms that the complexes are cell cycle regulated. We also show that the cyclin A protein is a component of the S phase complex. Each complex can be dissociated by the adenovirus E1A 12S product, releasing free E2F. The release of E2F from the cyclin A complex coincides with the stimulation of an E2F-dependent promoter. We suggest that these interactions control the activity of E2F and that disruption of the complexes by E1A contributes to a loss of cellular proliferation control.

Original languageEnglish (US)
Pages (from-to)1243-1253
Number of pages11
JournalCell
Volume65
Issue number7
DOIs
StatePublished - Jun 28 1991
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Mudryj, M., Devoto, S. H., Hiebert, S. W., Hunter, T., Pines, J., & Nevins, J. R. (1991). Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A. Cell, 65(7), 1243-1253. https://doi.org/10.1016/0092-8674(91)90019-U