Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A

Maria Mudryj, Stephen H. Devoto, Scott W. Hiebert, Tony Hunter, Jonathon Pines, Joseph R. Nevins

Research output: Contribution to journalArticle

211 Citations (Scopus)

Abstract

We have examined E2F binding activity in extracts of synchronizedNIH 3T3 cells. During the G0 to G1 transition, there is a marked increase in the level of active E2F. Subsequently, there are changes in the nature of E2F-containing complexes. A G1-specific complex increases in abundance, disappears, and is then replaced by another complex as S phase begins. Analysis of extracts of thymidine-blocked cells confirms that the complexes are cell cycle regulated. We also show that the cyclin A protein is a component of the S phase complex. Each complex can be dissociated by the adenovirus E1A 12S product, releasing free E2F. The release of E2F from the cyclin A complex coincides with the stimulation of an E2F-dependent promoter. We suggest that these interactions control the activity of E2F and that disruption of the complexes by E1A contributes to a loss of cellular proliferation control.

Original languageEnglish (US)
Pages (from-to)1243-1253
Number of pages11
JournalCell
Volume65
Issue number7
DOIs
StatePublished - Jun 28 1991
Externally publishedYes

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E2F Transcription Factors
Cyclin A
S Phase
Cell Cycle
Cells
3T3 Cells
Adenoviridae
Thymidine
Cell Proliferation
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Mudryj, M., Devoto, S. H., Hiebert, S. W., Hunter, T., Pines, J., & Nevins, J. R. (1991). Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A. Cell, 65(7), 1243-1253. https://doi.org/10.1016/0092-8674(91)90019-U

Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A. / Mudryj, Maria; Devoto, Stephen H.; Hiebert, Scott W.; Hunter, Tony; Pines, Jonathon; Nevins, Joseph R.

In: Cell, Vol. 65, No. 7, 28.06.1991, p. 1243-1253.

Research output: Contribution to journalArticle

Mudryj, M, Devoto, SH, Hiebert, SW, Hunter, T, Pines, J & Nevins, JR 1991, 'Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A', Cell, vol. 65, no. 7, pp. 1243-1253. https://doi.org/10.1016/0092-8674(91)90019-U
Mudryj, Maria ; Devoto, Stephen H. ; Hiebert, Scott W. ; Hunter, Tony ; Pines, Jonathon ; Nevins, Joseph R. / Cell cycle regulation of the E2F transcription factor involves an interaction with cyclin A. In: Cell. 1991 ; Vol. 65, No. 7. pp. 1243-1253.
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