Cell Cycle Regulation by Galectin-12, a New Member of the Galectin Superfamily

Ri Yao Yang, Daniel K. Hsu, Lan Yu, Jian Ni, Fu-Tong Liu

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Galectins are a family of β-galactoside-binding animal lectins with conserved carbohydrate recognition domains (CRDs). Here we report the identification and characterization of a new galectin, galectin-12, which contains two domains that are homologous to the galectin CRD. The N-terminal domain contains all of the sequence elements predicted to form the two β-sheets found in other galectins, as well as conserved carbohydrate-interacting residues. The C-terminal domain shows considerable divergence from the consensus sequence, and many of these conserved residues are not present. Nevertheless, the protein has lactose binding activity, most likely due to the contribution of the N-terminal domain. The mRNA for galectin-12 contains features coding for proteins with growth-regulatory functions. These include start codons in a context that are suboptimal for translation initiation and AU-rich motifs in the 3′-untranslated region, which are known to confer instability to mRNA. Galectin-12 mRNA is sparingly expressed or undetectable in many tissues and cell lines tested, but it is up-regulated in cells synchronized at the G1 phase or the G 1/S boundary of the cell cycle. Ectopic expression of galectin-12 in cancer cells causes cell cycle arrest at the G1 phase and cell growth suppression. We conclude that galectin-12 is a novel regulator of cellular homeostasis.

Original languageEnglish (US)
Pages (from-to)20252-20260
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number23
DOIs
StatePublished - Jun 8 2001

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Galectins
Cell Cycle
Cells
Carbohydrates
G1 Phase
Messenger RNA
Galactosides
Initiator Codon
RNA Stability
Consensus Sequence
Cell growth
3' Untranslated Regions
Lactose
Growth
Cell Cycle Checkpoints
Lectins
Proteins
Homeostasis
Tissue
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cell Cycle Regulation by Galectin-12, a New Member of the Galectin Superfamily. / Yang, Ri Yao; Hsu, Daniel K.; Yu, Lan; Ni, Jian; Liu, Fu-Tong.

In: Journal of Biological Chemistry, Vol. 276, No. 23, 08.06.2001, p. 20252-20260.

Research output: Contribution to journalArticle

Yang, Ri Yao ; Hsu, Daniel K. ; Yu, Lan ; Ni, Jian ; Liu, Fu-Tong. / Cell Cycle Regulation by Galectin-12, a New Member of the Galectin Superfamily. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 23. pp. 20252-20260.
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