Cell cycle phosphorylation of mitotic exit network (MEN) proteins

Michele H. Jones, Jamie M. Keck, Catherine C.L. Wong, Tao Xu, John R. Yates, Mark Winey

Research output: Contribution to journalReview article

8 Citations (Scopus)

Abstract

Phosphorylation of proteins is an important mechanism used to regulate most cellular processes. Recently we completed an extensive phosphoproteomic analysis of the core proteins that constitute the Saccharomyces cerevisiae centrosome. Here we present a study of phosphorylation sites found on the mitotic exit network (MEN) proteins, most of which are associated with the cytoplasmic face of the centrosome. We identified 55 sites on Bfa1, Cdc5, Cdc14 and Cdc15. Eight sites lie in cyclin-dependent kinase motifs (Cdk, S/T-P), and 22 sites are completely conserved within fungi. More than half of the sites were found in centrosomes from mitotic cells, possibly in preparation for their roles in mitotic exit. Finally, we report phosphorylation site information for other important cell cycle and regulatory proteins.

Original languageEnglish (US)
Pages (from-to)3435-3440
Number of pages6
JournalCell Cycle
Volume10
Issue number20
DOIs
StatePublished - Oct 31 2011
Externally publishedYes

Fingerprint

Centrosome
Cell Cycle
Phosphorylation
Saccharomyces cerevisiae Proteins
Cell Cycle Proteins
Proteins
Cyclin-Dependent Kinases
Fungi

Keywords

  • Cdk (cyclin-dependent kinase)/Cdc28
  • Cell cycle
  • In vivo phosphorylation
  • Mitotic exit network (MEN)
  • Plk1 (polo-like kinase)/Cdc5
  • Protein kinase
  • Yeast centrosome

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Jones, M. H., Keck, J. M., Wong, C. C. L., Xu, T., Yates, J. R., & Winey, M. (2011). Cell cycle phosphorylation of mitotic exit network (MEN) proteins. Cell Cycle, 10(20), 3435-3440. https://doi.org/10.4161/cc.10.20.17790

Cell cycle phosphorylation of mitotic exit network (MEN) proteins. / Jones, Michele H.; Keck, Jamie M.; Wong, Catherine C.L.; Xu, Tao; Yates, John R.; Winey, Mark.

In: Cell Cycle, Vol. 10, No. 20, 31.10.2011, p. 3435-3440.

Research output: Contribution to journalReview article

Jones, MH, Keck, JM, Wong, CCL, Xu, T, Yates, JR & Winey, M 2011, 'Cell cycle phosphorylation of mitotic exit network (MEN) proteins', Cell Cycle, vol. 10, no. 20, pp. 3435-3440. https://doi.org/10.4161/cc.10.20.17790
Jones, Michele H. ; Keck, Jamie M. ; Wong, Catherine C.L. ; Xu, Tao ; Yates, John R. ; Winey, Mark. / Cell cycle phosphorylation of mitotic exit network (MEN) proteins. In: Cell Cycle. 2011 ; Vol. 10, No. 20. pp. 3435-3440.
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