Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast

Kazuhiro Shiozaki, Paul Russell

Research output: Contribution to journalArticle

366 Citations (Scopus)

Abstract

In fission yeast the onset of mitosis is brought about by Cdc2/Cdc13 kinase, which is inhibited by the Wee1/Mik1 tyrosine kinases and activated by Cdc25 tyrosine phosphatase. This control network integrates many signals, including those that monitor DNA replication, DNA damage and cell size. We report here that a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Fission yeast spc1- mutants have a G2 delay that is greatly exacerbated by growth in high osmolarity media and nutrient limitation. A lethal interaction of spc1 and cdc25 mutations shows that Spc1 promotes the onset of mitosis. Spc1 is a MAP kinase homologue that is activated by Wis1 kinase in response to osmotic stress and nutrient limitation. Spc1 is inactivated by Pyp1, a phosphatase previously identified as a mitotic inhibitor. Pyp1 dephosphorylates only tyrosine-173 of Spc1, unlike the dual-specificity phosphatases that have been shown to regulate other MAP kinases.

Original languageEnglish (US)
Pages (from-to)739-743
Number of pages5
JournalNature
Volume378
Issue number6558
DOIs
StatePublished - Dec 14 1995
Externally publishedYes

Fingerprint

Schizosaccharomyces
Cell Cycle Checkpoints
Phosphotransferases
Mitosis
Tyrosine
cdc25 Phosphatases
Dual-Specificity Phosphatases
G2 Phase Cell Cycle Checkpoints
Osmoregulation
Food
Cell Physiological Phenomena
DNA Replication
Cell Size
Phosphoric Monoester Hydrolases
Protein-Tyrosine Kinases
Osmolar Concentration
DNA Damage
Mutation
Growth

ASJC Scopus subject areas

  • General

Cite this

Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast. / Shiozaki, Kazuhiro; Russell, Paul.

In: Nature, Vol. 378, No. 6558, 14.12.1995, p. 739-743.

Research output: Contribution to journalArticle

Shiozaki, Kazuhiro ; Russell, Paul. / Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast. In: Nature. 1995 ; Vol. 378, No. 6558. pp. 739-743.
@article{65f55570237445f2985f2813958448d2,
title = "Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast",
abstract = "In fission yeast the onset of mitosis is brought about by Cdc2/Cdc13 kinase, which is inhibited by the Wee1/Mik1 tyrosine kinases and activated by Cdc25 tyrosine phosphatase. This control network integrates many signals, including those that monitor DNA replication, DNA damage and cell size. We report here that a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Fission yeast spc1- mutants have a G2 delay that is greatly exacerbated by growth in high osmolarity media and nutrient limitation. A lethal interaction of spc1 and cdc25 mutations shows that Spc1 promotes the onset of mitosis. Spc1 is a MAP kinase homologue that is activated by Wis1 kinase in response to osmotic stress and nutrient limitation. Spc1 is inactivated by Pyp1, a phosphatase previously identified as a mitotic inhibitor. Pyp1 dephosphorylates only tyrosine-173 of Spc1, unlike the dual-specificity phosphatases that have been shown to regulate other MAP kinases.",
author = "Kazuhiro Shiozaki and Paul Russell",
year = "1995",
month = "12",
day = "14",
doi = "10.1038/378739a0",
language = "English (US)",
volume = "378",
pages = "739--743",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6558",

}

TY - JOUR

T1 - Cell-cycle control linked to extracellular environment by MAP kinase pathway in fission yeast

AU - Shiozaki, Kazuhiro

AU - Russell, Paul

PY - 1995/12/14

Y1 - 1995/12/14

N2 - In fission yeast the onset of mitosis is brought about by Cdc2/Cdc13 kinase, which is inhibited by the Wee1/Mik1 tyrosine kinases and activated by Cdc25 tyrosine phosphatase. This control network integrates many signals, including those that monitor DNA replication, DNA damage and cell size. We report here that a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Fission yeast spc1- mutants have a G2 delay that is greatly exacerbated by growth in high osmolarity media and nutrient limitation. A lethal interaction of spc1 and cdc25 mutations shows that Spc1 promotes the onset of mitosis. Spc1 is a MAP kinase homologue that is activated by Wis1 kinase in response to osmotic stress and nutrient limitation. Spc1 is inactivated by Pyp1, a phosphatase previously identified as a mitotic inhibitor. Pyp1 dephosphorylates only tyrosine-173 of Spc1, unlike the dual-specificity phosphatases that have been shown to regulate other MAP kinases.

AB - In fission yeast the onset of mitosis is brought about by Cdc2/Cdc13 kinase, which is inhibited by the Wee1/Mik1 tyrosine kinases and activated by Cdc25 tyrosine phosphatase. This control network integrates many signals, including those that monitor DNA replication, DNA damage and cell size. We report here that a fission yeast MAP kinase pathway links the cell-cycle G2/M control with changes in the extracellular environment that affect cell physiology. Fission yeast spc1- mutants have a G2 delay that is greatly exacerbated by growth in high osmolarity media and nutrient limitation. A lethal interaction of spc1 and cdc25 mutations shows that Spc1 promotes the onset of mitosis. Spc1 is a MAP kinase homologue that is activated by Wis1 kinase in response to osmotic stress and nutrient limitation. Spc1 is inactivated by Pyp1, a phosphatase previously identified as a mitotic inhibitor. Pyp1 dephosphorylates only tyrosine-173 of Spc1, unlike the dual-specificity phosphatases that have been shown to regulate other MAP kinases.

UR - http://www.scopus.com/inward/record.url?scp=0029563651&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029563651&partnerID=8YFLogxK

U2 - 10.1038/378739a0

DO - 10.1038/378739a0

M3 - Article

VL - 378

SP - 739

EP - 743

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6558

ER -