Cell autonomous and nonautonomous requirements for Delltalike1 during early mouse retinal neurogenesis

Amy N. Riesenberg, Nadean L Brown

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: In the vertebrate retina, six neuronal and one glial cell class are produced from a common progenitor pool. During neurogenesis, adjacent retinal cells use Notch signaling to maintain a pool of progenitors by blocking particular cells from differentiating prematurely. In mice there are multiple Notch pathway ligands and receptors, but the role(s) of each paralogue during retinal histogenesis remains only partially defined. Results: Here we analyzed the cell autonomous and nonautonomous requirements for the Deltalike1(Dll1) ligand during prenatal retinogenesis. We used the α-Cre driver to simultaneously delete a Dll1 conditional allele and activate the Z/EG reporter, then quantified Dll1 mutant phenotypes within and outside of this α-Cre GFP-marked lineage. We found that Dll1 activity is required for Hes1 expression, both autonomously and nonautonomously, but were surprised that retinal ganglion cell differentiation is only blocked cell autonomously. Moreover, Dll1 does not act during cone photoreceptor neurogenesis. Finally, Dll1 mutant adult retinas contained small retinal rosettes and RGC patterning defects but were otherwise normal. Conclusions: Although Dll1 participates in bidirectional (cis + trans) Notch signaling to regulate Hes1 expression, it only acts cell autonomously (in cis) to interpret inhibitory signals from other cells that block RGC neurogenesis. Developmental Dynamics 245:631-640, 2016. Key findings: Retinal progenitors require Dll1 both in cis and trans RGC neurogenesis requires Dll1 in cis. Dll1 does not regulate cone photoreceptor development.

Original languageEnglish (US)
Pages (from-to)631-640
Number of pages10
JournalDevelopmental Dynamics
Volume245
Issue number6
DOIs
StatePublished - Jun 1 2016

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Neurogenesis
Retinal Cone Photoreceptor Cells
Retina
Ligands
Retinal Ganglion Cells
Neuroglia
Vertebrates
Cell Differentiation
Alleles
Phenotype

Keywords

  • Cone photoreceptor
  • Lateral inhibition
  • Notch signaling
  • Retinal ganglion cell
  • Retinogenesis

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Cell autonomous and nonautonomous requirements for Delltalike1 during early mouse retinal neurogenesis. / Riesenberg, Amy N.; Brown, Nadean L.

In: Developmental Dynamics, Vol. 245, No. 6, 01.06.2016, p. 631-640.

Research output: Contribution to journalArticle

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