Abstract
Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 609-613 |
| Number of pages | 5 |
| Journal | Avian Diseases |
| Volume | 49 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2005 |
Keywords
- Brachyspira pilosicoli
- Ceca
- Pathology
- PCR
- Turkeys
ASJC Scopus subject areas
- Immunology
- Cancer Research
- veterinary(all)