Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys

H L Shivaprasad, G. E. Duhamel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.

Original languageEnglish (US)
Pages (from-to)609-613
Number of pages5
JournalAvian Diseases
Volume49
Issue number4
DOIs
StatePublished - Dec 2005

Fingerprint

Brachyspira
Brachyspira pilosicoli
Cecum
Typhlitis
Epithelium
16S Ribosomal RNA
Laser Capture Microdissection
Spirochaetales
rRNA Genes
cecum
epithelium
Monoclonal Antibodies
Staining and Labeling
Polymerase Chain Reaction
DNA
lasers
monoclonal antibodies
polymerase chain reaction
ribosomal RNA
microorganisms

Keywords

  • Brachyspira pilosicoli
  • Ceca
  • Pathology
  • PCR
  • Turkeys

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

Cite this

Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys. / Shivaprasad, H L; Duhamel, G. E.

In: Avian Diseases, Vol. 49, No. 4, 12.2005, p. 609-613.

Research output: Contribution to journalArticle

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