Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys

H L Shivaprasad, G. E. Duhamel

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.

Original languageEnglish (US)
Pages (from-to)609-613
Number of pages5
JournalAvian Diseases
Volume49
Issue number4
DOIs
StatePublished - Dec 2005

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Keywords

  • Brachyspira pilosicoli
  • Ceca
  • Pathology
  • PCR
  • Turkeys

ASJC Scopus subject areas

  • Immunology
  • Cancer Research
  • veterinary(all)

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