Abstract
Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 609-613 |
| Number of pages | 5 |
| Journal | Avian Diseases |
| Volume | 49 |
| Issue number | 4 |
| DOIs | |
| State | Published - Dec 2005 |
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Keywords
- Brachyspira pilosicoli
- Ceca
- Pathology
- PCR
- Turkeys
ASJC Scopus subject areas
- Immunology
- Cancer Research
- veterinary(all)
Cite this
Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys. / Shivaprasad, H L; Duhamel, G. E.
In: Avian Diseases, Vol. 49, No. 4, 12.2005, p. 609-613.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cecal spirochetosis caused by Brachyspira pilosicoli in commercial turkeys
AU - Shivaprasad, H L
AU - Duhamel, G. E.
PY - 2005/12
Y1 - 2005/12
N2 - Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
AB - Spirochetes that were identified as Brachyspira pilosicoli were present in the ceca of 7.5- to 18-wk-old turkeys with cecal spirochetosis and typhlitis. The identity of B. pilosicoli was confirmed on the basis of ultrastructural morphology of the cecal epithelium adherent microbes, immunohistochemical staining with a Brachyspira genus-specific monoclonal antibody, and amplification of a B. pilosicoli species-specific 16S ribosomal RNA (rrs gene) sequence by using the polymerase chain reaction and DNA obtained by laser-capture microdissection of the epithelium-adherent microbial fringe. To the author's knowledge, this is the first report of B. pilosicoli in the ceca of turkeys.
KW - Brachyspira pilosicoli
KW - Ceca
KW - Pathology
KW - PCR
KW - Turkeys
UR - http://www.scopus.com/inward/record.url?scp=29244439911&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29244439911&partnerID=8YFLogxK
U2 - 10.1637/7383-052005.1
DO - 10.1637/7383-052005.1
M3 - Article
C2 - 16405009
AN - SCOPUS:29244439911
VL - 49
SP - 609
EP - 613
JO - Avian Diseases
JF - Avian Diseases
SN - 0005-2086
IS - 4
ER -