cDNA Cloning and Expression of a Soluble Epoxide Hydrolase from Human Liver

J. K. Beetham, T. G. Tian, B. D. Hammock

Research output: Contribution to journalArticlepeer-review

134 Scopus citations

Abstract

We report the cloning and expression of a cDNA that encodes a soluble epoxide hydrolase from human liver. The 2101-base clone predicts a 554-residue protein (Mr 62,640) with an apparently imperfect peroxisomal targeting signal of Ser-Lys-Met at the carboxy terminus. The cDNA was expressed in the baculovirus system in the Spodoptera frugiperda 21 cell line. The recombinant protein was similar to soluble epoxide hydrolase isolated from human liver in terms of molecular weight, hydrolytic activity, inhibition, and immunoreactivity.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume305
Issue number1
DOIs
StatePublished - Aug 15 1993

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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