CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools

Nicole Baumgarth, Gina C. Jager, Ometa C. Herman, Leonard A. Herzenberg, Leonore A. Herzenberg

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We demonstrate that adoptive transfer of peritoneal cavity B cells fails to replenish the peripheral B-1 cells in adult B cell-deficient (μ(-/-)) mice but does replenish adult RAG-1(-/-) mice. We show that this lack of self-replenishment in μ(-/-) mice is mediated by strongly inhibitory, radiation-sensitive CD4+ T cells that also function in cotransfer studies to block the reconstitution of B-1 cells and inhibit accumulation of bone marrow-derived B-2 cells in the periphery in irradiated recipients. CD8+ T cells from μ(-/-) do not mediate this inhibition. The inhibitory CD4+ T cells develop early in life, because B-1 cell replenishment occurs normally when B-1 cells are transferred into μ(-/-) neonates. Thus, we conclude that the presence of B cells in the neonate conditions the CD4+ T-cell population to permit the establishment and maintenance of normal B cell pools throughout life.

Original languageEnglish (US)
Pages (from-to)4766-4771
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number9
DOIs
StatePublished - Apr 25 2000
Externally publishedYes

Fingerprint

B-Lymphocytes
T-Lymphocytes
Adoptive Transfer
Peritoneal Cavity
Bone Marrow
Maintenance
Radiation
Population

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools. / Baumgarth, Nicole; Jager, Gina C.; Herman, Ometa C.; Herzenberg, Leonard A.; Herzenberg, Leonore A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 9, 25.04.2000, p. 4766-4771.

Research output: Contribution to journalArticle

Baumgarth, Nicole ; Jager, Gina C. ; Herman, Ometa C. ; Herzenberg, Leonard A. ; Herzenberg, Leonore A. / CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 9. pp. 4766-4771.
@article{a1cd6c34c9b24dbba8125295f0640ac4,
title = "CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools",
abstract = "We demonstrate that adoptive transfer of peritoneal cavity B cells fails to replenish the peripheral B-1 cells in adult B cell-deficient (μ(-/-)) mice but does replenish adult RAG-1(-/-) mice. We show that this lack of self-replenishment in μ(-/-) mice is mediated by strongly inhibitory, radiation-sensitive CD4+ T cells that also function in cotransfer studies to block the reconstitution of B-1 cells and inhibit accumulation of bone marrow-derived B-2 cells in the periphery in irradiated recipients. CD8+ T cells from μ(-/-) do not mediate this inhibition. The inhibitory CD4+ T cells develop early in life, because B-1 cell replenishment occurs normally when B-1 cells are transferred into μ(-/-) neonates. Thus, we conclude that the presence of B cells in the neonate conditions the CD4+ T-cell population to permit the establishment and maintenance of normal B cell pools throughout life.",
author = "Nicole Baumgarth and Jager, {Gina C.} and Herman, {Ometa C.} and Herzenberg, {Leonard A.} and Herzenberg, {Leonore A.}",
year = "2000",
month = "4",
day = "25",
doi = "10.1073/pnas.97.9.4766",
language = "English (US)",
volume = "97",
pages = "4766--4771",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "9",

}

TY - JOUR

T1 - CD4+ T cells derived from B cell-deficient mice inhibit the establishment of peripheral B cell pools

AU - Baumgarth, Nicole

AU - Jager, Gina C.

AU - Herman, Ometa C.

AU - Herzenberg, Leonard A.

AU - Herzenberg, Leonore A.

PY - 2000/4/25

Y1 - 2000/4/25

N2 - We demonstrate that adoptive transfer of peritoneal cavity B cells fails to replenish the peripheral B-1 cells in adult B cell-deficient (μ(-/-)) mice but does replenish adult RAG-1(-/-) mice. We show that this lack of self-replenishment in μ(-/-) mice is mediated by strongly inhibitory, radiation-sensitive CD4+ T cells that also function in cotransfer studies to block the reconstitution of B-1 cells and inhibit accumulation of bone marrow-derived B-2 cells in the periphery in irradiated recipients. CD8+ T cells from μ(-/-) do not mediate this inhibition. The inhibitory CD4+ T cells develop early in life, because B-1 cell replenishment occurs normally when B-1 cells are transferred into μ(-/-) neonates. Thus, we conclude that the presence of B cells in the neonate conditions the CD4+ T-cell population to permit the establishment and maintenance of normal B cell pools throughout life.

AB - We demonstrate that adoptive transfer of peritoneal cavity B cells fails to replenish the peripheral B-1 cells in adult B cell-deficient (μ(-/-)) mice but does replenish adult RAG-1(-/-) mice. We show that this lack of self-replenishment in μ(-/-) mice is mediated by strongly inhibitory, radiation-sensitive CD4+ T cells that also function in cotransfer studies to block the reconstitution of B-1 cells and inhibit accumulation of bone marrow-derived B-2 cells in the periphery in irradiated recipients. CD8+ T cells from μ(-/-) do not mediate this inhibition. The inhibitory CD4+ T cells develop early in life, because B-1 cell replenishment occurs normally when B-1 cells are transferred into μ(-/-) neonates. Thus, we conclude that the presence of B cells in the neonate conditions the CD4+ T-cell population to permit the establishment and maintenance of normal B cell pools throughout life.

UR - http://www.scopus.com/inward/record.url?scp=0034712736&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034712736&partnerID=8YFLogxK

U2 - 10.1073/pnas.97.9.4766

DO - 10.1073/pnas.97.9.4766

M3 - Article

C2 - 10781082

AN - SCOPUS:0034712736

VL - 97

SP - 4766

EP - 4771

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 9

ER -