We demonstrate that adoptive transfer of peritoneal cavity B cells fails to replenish the peripheral B-1 cells in adult B cell-deficient (μ(-/-)) mice but does replenish adult RAG-1(-/-) mice. We show that this lack of self-replenishment in μ(-/-) mice is mediated by strongly inhibitory, radiation-sensitive CD4+ T cells that also function in cotransfer studies to block the reconstitution of B-1 cells and inhibit accumulation of bone marrow-derived B-2 cells in the periphery in irradiated recipients. CD8+ T cells from μ(-/-) do not mediate this inhibition. The inhibitory CD4+ T cells develop early in life, because B-1 cell replenishment occurs normally when B-1 cells are transferred into μ(-/-) neonates. Thus, we conclude that the presence of B cells in the neonate conditions the CD4+ T-cell population to permit the establishment and maintenance of normal B cell pools throughout life.
|Original language||English (US)|
|Number of pages||6|
|Journal||Proceedings of the National Academy of Sciences of the United States of America|
|State||Published - Apr 25 2000|
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