CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs

Guo Xiang Yang, Zhe Xiong Lian, Kentaro Kikuchi, Yong Jun Liu, Aftab A. Ansari, Susumu Ikehara, M. Eric Gershwin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We have recently identified two groups of plasmacytoid dendritic cells (pDCs) isolated from murine liver based on the expression of CD4 and other cell surface markers uniquely expressed by pDCs. Herein, we describe the identification of both CD4+ and CD4- pDCs that clearly exist in lymph nodes (LNs), spleen, liver, thymus, bone marrow, and lung. Normally, CD4+ pDCs are enriched in LNs. However, after in vivo systemic injection with bacterial CpG, a larger number of CD4- pDCs are recruited to the LNs and local inoculation by CpG drives CD4- pDCs migrating into local sentinel LNs, suggesting that CD4- pDCs are the main subpopulation migrating to the peripheral LNs. Furthermore, although both freshly isolated CD4+ pDCs and CD4- pDCs appear as an immature plasmacytoid cell and develop into a DC morphology following activation, the two subsets have strikingly different immune features, including differences in the production pattern of cytokines stimulated with CpG and in T cell activation.

Original languageEnglish (US)
Pages (from-to)3197-3203
Number of pages7
JournalJournal of Immunology
Volume174
Issue number6
StatePublished - Mar 15 2005

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Dendritic Cells
Lymph Nodes
Liver
Thymus Gland
Spleen
Bone Marrow
Cytokines
T-Lymphocytes
Lung
Injections

ASJC Scopus subject areas

  • Immunology

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CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs. / Yang, Guo Xiang; Lian, Zhe Xiong; Kikuchi, Kentaro; Liu, Yong Jun; Ansari, Aftab A.; Ikehara, Susumu; Gershwin, M. Eric.

In: Journal of Immunology, Vol. 174, No. 6, 15.03.2005, p. 3197-3203.

Research output: Contribution to journalArticle

Yang, Guo Xiang ; Lian, Zhe Xiong ; Kikuchi, Kentaro ; Liu, Yong Jun ; Ansari, Aftab A. ; Ikehara, Susumu ; Gershwin, M. Eric. / CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs. In: Journal of Immunology. 2005 ; Vol. 174, No. 6. pp. 3197-3203.
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AU - Kikuchi, Kentaro

AU - Liu, Yong Jun

AU - Ansari, Aftab A.

AU - Ikehara, Susumu

AU - Gershwin, M. Eric

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