Abstract
We have recently identified two groups of plasmacytoid dendritic cells (pDCs) isolated from murine liver based on the expression of CD4 and other cell surface markers uniquely expressed by pDCs. Herein, we describe the identification of both CD4+ and CD4- pDCs that clearly exist in lymph nodes (LNs), spleen, liver, thymus, bone marrow, and lung. Normally, CD4+ pDCs are enriched in LNs. However, after in vivo systemic injection with bacterial CpG, a larger number of CD4- pDCs are recruited to the LNs and local inoculation by CpG drives CD4- pDCs migrating into local sentinel LNs, suggesting that CD4- pDCs are the main subpopulation migrating to the peripheral LNs. Furthermore, although both freshly isolated CD4+ pDCs and CD4- pDCs appear as an immature plasmacytoid cell and develop into a DC morphology following activation, the two subsets have strikingly different immune features, including differences in the production pattern of cytokines stimulated with CpG and in T cell activation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3197-3203 |
| Number of pages | 7 |
| Journal | Journal of Immunology |
| Volume | 174 |
| Issue number | 6 |
| State | Published - Mar 15 2005 |
Fingerprint
ASJC Scopus subject areas
- Immunology
Cite this
CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs. / Yang, Guo Xiang; Lian, Zhe Xiong; Kikuchi, Kentaro; Liu, Yong Jun; Ansari, Aftab A.; Ikehara, Susumu; Gershwin, M. Eric.
In: Journal of Immunology, Vol. 174, No. 6, 15.03.2005, p. 3197-3203.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD4- plasmacytoid dendritic cells (pDCs) migrate in lymph nodes by CpG inoculation and represent a potent functional subset of pDCs
AU - Yang, Guo Xiang
AU - Lian, Zhe Xiong
AU - Kikuchi, Kentaro
AU - Liu, Yong Jun
AU - Ansari, Aftab A.
AU - Ikehara, Susumu
AU - Gershwin, M. Eric
PY - 2005/3/15
Y1 - 2005/3/15
N2 - We have recently identified two groups of plasmacytoid dendritic cells (pDCs) isolated from murine liver based on the expression of CD4 and other cell surface markers uniquely expressed by pDCs. Herein, we describe the identification of both CD4+ and CD4- pDCs that clearly exist in lymph nodes (LNs), spleen, liver, thymus, bone marrow, and lung. Normally, CD4+ pDCs are enriched in LNs. However, after in vivo systemic injection with bacterial CpG, a larger number of CD4- pDCs are recruited to the LNs and local inoculation by CpG drives CD4- pDCs migrating into local sentinel LNs, suggesting that CD4- pDCs are the main subpopulation migrating to the peripheral LNs. Furthermore, although both freshly isolated CD4+ pDCs and CD4- pDCs appear as an immature plasmacytoid cell and develop into a DC morphology following activation, the two subsets have strikingly different immune features, including differences in the production pattern of cytokines stimulated with CpG and in T cell activation.
AB - We have recently identified two groups of plasmacytoid dendritic cells (pDCs) isolated from murine liver based on the expression of CD4 and other cell surface markers uniquely expressed by pDCs. Herein, we describe the identification of both CD4+ and CD4- pDCs that clearly exist in lymph nodes (LNs), spleen, liver, thymus, bone marrow, and lung. Normally, CD4+ pDCs are enriched in LNs. However, after in vivo systemic injection with bacterial CpG, a larger number of CD4- pDCs are recruited to the LNs and local inoculation by CpG drives CD4- pDCs migrating into local sentinel LNs, suggesting that CD4- pDCs are the main subpopulation migrating to the peripheral LNs. Furthermore, although both freshly isolated CD4+ pDCs and CD4- pDCs appear as an immature plasmacytoid cell and develop into a DC morphology following activation, the two subsets have strikingly different immune features, including differences in the production pattern of cytokines stimulated with CpG and in T cell activation.
UR - http://www.scopus.com/inward/record.url?scp=14844357925&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14844357925&partnerID=8YFLogxK
M3 - Article
C2 - 15749849
AN - SCOPUS:14844357925
VL - 174
SP - 3197
EP - 3203
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -