CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalomyelitis

Sara Abromson-Leeman, Emanual Michael Maverakis, Roderick Bronson, Martin E. Dorf

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.

Original languageEnglish (US)
Pages (from-to)527-538
Number of pages12
JournalEuropean Journal of Immunology
Volume31
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Myelin Basic Protein
Autoimmune Experimental Encephalomyelitis
T-Lymphocytes
Th1 Cells
Central Nervous System
CD40 Ligand
Central Nervous System Diseases
Interleukin-12
Myelin Sheath
Blood-Brain Barrier
Epitopes

Keywords

  • CD40
  • Cellular activation
  • Experimental autoimmune encephalomyelitis
  • IL-12
  • In vivo animal model

ASJC Scopus subject areas

  • Immunology

Cite this

@article{2a1061e9424c487a88e80c8b2830766e,
title = "CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalomyelitis",
abstract = "CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.",
keywords = "CD40, Cellular activation, Experimental autoimmune encephalomyelitis, IL-12, In vivo animal model",
author = "Sara Abromson-Leeman and Maverakis, {Emanual Michael} and Roderick Bronson and Dorf, {Martin E.}",
year = "2001",
doi = "10.1002/1521-4141(200102)31:2<527::AID-IMMU527>3.0.CO;2-D",
language = "English (US)",
volume = "31",
pages = "527--538",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "2",

}

TY - JOUR

T1 - CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalomyelitis

AU - Abromson-Leeman, Sara

AU - Maverakis, Emanual Michael

AU - Bronson, Roderick

AU - Dorf, Martin E.

PY - 2001

Y1 - 2001

N2 - CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.

AB - CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.

KW - CD40

KW - Cellular activation

KW - Experimental autoimmune encephalomyelitis

KW - IL-12

KW - In vivo animal model

UR - http://www.scopus.com/inward/record.url?scp=0035101312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035101312&partnerID=8YFLogxK

U2 - 10.1002/1521-4141(200102)31:2<527::AID-IMMU527>3.0.CO;2-D

DO - 10.1002/1521-4141(200102)31:2<527::AID-IMMU527>3.0.CO;2-D

M3 - Article

VL - 31

SP - 527

EP - 538

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 2

ER -