Abstract
CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 527-538 |
| Number of pages | 12 |
| Journal | European Journal of Immunology |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| State | Published - 2001 |
| Externally published | Yes |
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Keywords
- CD40
- Cellular activation
- Experimental autoimmune encephalomyelitis
- IL-12
- In vivo animal model
ASJC Scopus subject areas
- Immunology
Cite this
CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalomyelitis. / Abromson-Leeman, Sara; Maverakis, Emanual Michael; Bronson, Roderick; Dorf, Martin E.
In: European Journal of Immunology, Vol. 31, No. 2, 2001, p. 527-538.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD40-mediated activation of T cells accelerates, but is not required for, encephalitogenic potential of myelin basic protein-recognizing T cells in a model of progressive experimental autoimmune encephalomyelitis
AU - Abromson-Leeman, Sara
AU - Maverakis, Emanual Michael
AU - Bronson, Roderick
AU - Dorf, Martin E.
PY - 2001
Y1 - 2001
N2 - CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.
AB - CD40 ligand-CD40 interactions are important in the development of experimental autoimmune encephalomyelitis (EAE), but it is unclear whether this interaction is critical for de novo recruitment of T cells, entry of T cells into the central nervous system (CNS), or effector function of T cells in vivo. In this report we define the role of CD40 in a model of progressive EAE that does not depend on epitope spread or recruitment of new myelin-specific T cells into the CNS. Results show that CD40 is not required for trans-migration of activated T cells through the endothelial blood-brain barrier, and in its absence T cells will both enter the CNS and induce disease. However, interaction with CD40 is critical for optimal activation and encephalitogenicity of cloned Th1 cells. In its presence, Th1 cells enter the CNS earlier and induce more severe disease. Inclusion of IL-12 during activation of Th1 cells in the absence of CD40 can override the otherwise suboptimal level of encephalitogenicity observed. The implication of these findings for theapeutic use of agents designed to block this pathway is discussed.
KW - CD40
KW - Cellular activation
KW - Experimental autoimmune encephalomyelitis
KW - IL-12
KW - In vivo animal model
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UR - http://www.scopus.com/inward/citedby.url?scp=0035101312&partnerID=8YFLogxK
U2 - 10.1002/1521-4141(200102)31:2<527::AID-IMMU527>3.0.CO;2-D
DO - 10.1002/1521-4141(200102)31:2<527::AID-IMMU527>3.0.CO;2-D
M3 - Article
VL - 31
SP - 527
EP - 538
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 2
ER -