CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides)

C. Brachelente, Verena K Affolter, A. Fondati, I. Porcellato, M. Sforna, E. Lepri, L. Mechelli, L. Bongiovanni

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A 14-year-old female spayed Dachshund was presented with generalized scaling, erythema, pruritus, poor quality of hair coat, and progressive weight loss. Cutaneous epitheliotropic T-cell lymphoma (CETCL) was suspected. Skin biopsies were suggestive of CETCL. However, immunohistochemistry revealed the presence of numerous CD20+ and CD3+ cells. Clonality assay demonstrated a clonal T-cell receptor gamma rearrangement and a polyclonal IgH gene rearrangement. Double-label immunofluorescence confirmed coexpression of CD3 and CD20 by neoplastic cells. By double immunohistochemistry, neoplastic cells were CD3+ and PAX5–. The results are compatible with a CD3+, CD20+ CETCL. Coexpression of CD20 and CD3 has been recognized in peripheral T-cell lymphomas. Although documented in human CETCL, it has not been reported in canine CETCL. The pathogenetic basis of CD20 expression in mycosis fungoides is explored.

Original languageEnglish (US)
Pages (from-to)563-566
Number of pages4
JournalVeterinary Pathology
Volume53
Issue number3
DOIs
StatePublished - 2015

Fingerprint

mycosis fungoides
Cutaneous T-Cell Lymphoma
Mycosis Fungoides
lymphoma
Canidae
T-lymphocytes
dogs
Immunohistochemistry
Peripheral T-Cell Lymphoma
immunohistochemistry
Gene Rearrangement
Erythema
Pruritus
T-Cell Antigen Receptor
Dachshund
Hair
Fluorescent Antibody Technique
erythema
Weight Loss
pruritus

Keywords

  • canine
  • CD20
  • CD3
  • clonality
  • cutaneous epitheliotropic T-cell lymphoma
  • double-label immunofluorescence
  • immunohistochemistry
  • PAX5
  • skin
  • T cell

ASJC Scopus subject areas

  • veterinary(all)

Cite this

CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides). / Brachelente, C.; Affolter, Verena K; Fondati, A.; Porcellato, I.; Sforna, M.; Lepri, E.; Mechelli, L.; Bongiovanni, L.

In: Veterinary Pathology, Vol. 53, No. 3, 2015, p. 563-566.

Research output: Contribution to journalArticle

Brachelente, C, Affolter, VK, Fondati, A, Porcellato, I, Sforna, M, Lepri, E, Mechelli, L & Bongiovanni, L 2015, 'CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides)', Veterinary Pathology, vol. 53, no. 3, pp. 563-566. https://doi.org/10.1177/0300985815604724
Brachelente, C. ; Affolter, Verena K ; Fondati, A. ; Porcellato, I. ; Sforna, M. ; Lepri, E. ; Mechelli, L. ; Bongiovanni, L. / CD3 and CD20 Coexpression in a Case of Canine Cutaneous Epitheliotropic T-Cell Lymphoma (Mycosis Fungoides). In: Veterinary Pathology. 2015 ; Vol. 53, No. 3. pp. 563-566.
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