CD14-mediated alterations in transcription and splicing of endogenous retroviruses after injury

Kiho Cho, T. N. Pham, T. Chamberlain, J. Boonyaratanakornkit, David G Greenhalgh

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Increase in systemic levels of lipopolysaccharide (LPS) contributes to the pathogenesis of distant organ injury after burn. Stress signals elicited from burn influence transcriptional activities of mouse endogenous retroviruses (MuERVs) in various distant organs. The involvement of LPS pathways in the burn-mediated regulation of MuERVs in the spleen was investigated in this study. Spleen harbors substantial numbers of tissue macrophages, a key responder to LPS stimulation. Spleen tissues collected from CD14 (LPS receptor) knockout (KO) and wild type (WT) mice after burn were subjected to RT-PCR analysis of MuERV expression. There was a substantial induction of 2 bands and a marked downregulation of a band in CD14 KO mice compared to WT mice after burn. Sequence analysis of these CD14- and burn-dependent bands identified 3 new alternatively spliced and 2 defective env transcripts of MuERVs as well as novel splicing signals. Chromosomal loci of putative MuERVs sharing the unique U3 sequences of these transcripts were mapped by surveying the entire genome of C57BL/6J mice. In addition, coding potentials, transcriptional regulatory elements, and adjacent cellular genes of these putative MuERVs were analyzed. The results from these studies suggest that injury-triggered LPS/CD14 signaling events play roles in the transcriptional regulation of certain MuERVs carrying unique U3 promoter sequences.

Original languageEnglish (US)
Pages (from-to)2215-2233
Number of pages19
JournalArchives of Virology
Volume149
Issue number11
DOIs
StatePublished - Nov 2004

Fingerprint

Endogenous Retroviruses
Wounds and Injuries
Lipopolysaccharides
Spleen
Burns
Knockout Mice
Transcriptional Regulatory Elements
CD14 Antigens
Inbred C57BL Mouse
Sequence Analysis
Down-Regulation
Macrophages
Genome
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Genetics
  • Applied Microbiology and Biotechnology

Cite this

CD14-mediated alterations in transcription and splicing of endogenous retroviruses after injury. / Cho, Kiho; Pham, T. N.; Chamberlain, T.; Boonyaratanakornkit, J.; Greenhalgh, David G.

In: Archives of Virology, Vol. 149, No. 11, 11.2004, p. 2215-2233.

Research output: Contribution to journalArticle

Cho, Kiho ; Pham, T. N. ; Chamberlain, T. ; Boonyaratanakornkit, J. ; Greenhalgh, David G. / CD14-mediated alterations in transcription and splicing of endogenous retroviruses after injury. In: Archives of Virology. 2004 ; Vol. 149, No. 11. pp. 2215-2233.
@article{d532b450ed9f4eb48cae97477776bf42,
title = "CD14-mediated alterations in transcription and splicing of endogenous retroviruses after injury",
abstract = "Increase in systemic levels of lipopolysaccharide (LPS) contributes to the pathogenesis of distant organ injury after burn. Stress signals elicited from burn influence transcriptional activities of mouse endogenous retroviruses (MuERVs) in various distant organs. The involvement of LPS pathways in the burn-mediated regulation of MuERVs in the spleen was investigated in this study. Spleen harbors substantial numbers of tissue macrophages, a key responder to LPS stimulation. Spleen tissues collected from CD14 (LPS receptor) knockout (KO) and wild type (WT) mice after burn were subjected to RT-PCR analysis of MuERV expression. There was a substantial induction of 2 bands and a marked downregulation of a band in CD14 KO mice compared to WT mice after burn. Sequence analysis of these CD14- and burn-dependent bands identified 3 new alternatively spliced and 2 defective env transcripts of MuERVs as well as novel splicing signals. Chromosomal loci of putative MuERVs sharing the unique U3 sequences of these transcripts were mapped by surveying the entire genome of C57BL/6J mice. In addition, coding potentials, transcriptional regulatory elements, and adjacent cellular genes of these putative MuERVs were analyzed. The results from these studies suggest that injury-triggered LPS/CD14 signaling events play roles in the transcriptional regulation of certain MuERVs carrying unique U3 promoter sequences.",
author = "Kiho Cho and Pham, {T. N.} and T. Chamberlain and J. Boonyaratanakornkit and Greenhalgh, {David G}",
year = "2004",
month = "11",
doi = "10.1007/s00705-004-0358-z",
language = "English (US)",
volume = "149",
pages = "2215--2233",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "11",

}

TY - JOUR

T1 - CD14-mediated alterations in transcription and splicing of endogenous retroviruses after injury

AU - Cho, Kiho

AU - Pham, T. N.

AU - Chamberlain, T.

AU - Boonyaratanakornkit, J.

AU - Greenhalgh, David G

PY - 2004/11

Y1 - 2004/11

N2 - Increase in systemic levels of lipopolysaccharide (LPS) contributes to the pathogenesis of distant organ injury after burn. Stress signals elicited from burn influence transcriptional activities of mouse endogenous retroviruses (MuERVs) in various distant organs. The involvement of LPS pathways in the burn-mediated regulation of MuERVs in the spleen was investigated in this study. Spleen harbors substantial numbers of tissue macrophages, a key responder to LPS stimulation. Spleen tissues collected from CD14 (LPS receptor) knockout (KO) and wild type (WT) mice after burn were subjected to RT-PCR analysis of MuERV expression. There was a substantial induction of 2 bands and a marked downregulation of a band in CD14 KO mice compared to WT mice after burn. Sequence analysis of these CD14- and burn-dependent bands identified 3 new alternatively spliced and 2 defective env transcripts of MuERVs as well as novel splicing signals. Chromosomal loci of putative MuERVs sharing the unique U3 sequences of these transcripts were mapped by surveying the entire genome of C57BL/6J mice. In addition, coding potentials, transcriptional regulatory elements, and adjacent cellular genes of these putative MuERVs were analyzed. The results from these studies suggest that injury-triggered LPS/CD14 signaling events play roles in the transcriptional regulation of certain MuERVs carrying unique U3 promoter sequences.

AB - Increase in systemic levels of lipopolysaccharide (LPS) contributes to the pathogenesis of distant organ injury after burn. Stress signals elicited from burn influence transcriptional activities of mouse endogenous retroviruses (MuERVs) in various distant organs. The involvement of LPS pathways in the burn-mediated regulation of MuERVs in the spleen was investigated in this study. Spleen harbors substantial numbers of tissue macrophages, a key responder to LPS stimulation. Spleen tissues collected from CD14 (LPS receptor) knockout (KO) and wild type (WT) mice after burn were subjected to RT-PCR analysis of MuERV expression. There was a substantial induction of 2 bands and a marked downregulation of a band in CD14 KO mice compared to WT mice after burn. Sequence analysis of these CD14- and burn-dependent bands identified 3 new alternatively spliced and 2 defective env transcripts of MuERVs as well as novel splicing signals. Chromosomal loci of putative MuERVs sharing the unique U3 sequences of these transcripts were mapped by surveying the entire genome of C57BL/6J mice. In addition, coding potentials, transcriptional regulatory elements, and adjacent cellular genes of these putative MuERVs were analyzed. The results from these studies suggest that injury-triggered LPS/CD14 signaling events play roles in the transcriptional regulation of certain MuERVs carrying unique U3 promoter sequences.

UR - http://www.scopus.com/inward/record.url?scp=8744251392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8744251392&partnerID=8YFLogxK

U2 - 10.1007/s00705-004-0358-z

DO - 10.1007/s00705-004-0358-z

M3 - Article

C2 - 15503208

AN - SCOPUS:8744251392

VL - 149

SP - 2215

EP - 2233

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 11

ER -