CD11c/CD18: Novel ligands and a role in delayed-type hypersensitivity

Chanchal Sadhu, Harold J. Ting, Brian Lipsky, Kelly Hensley, Leon F. Garcia-Martinez, Scott I. Simon, Donald E. Staunton

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

CD11c, a member of the leukointegrin family, is expressed prominently on tissue macrophages and dendritic cells and binds to complement fragment (iC3b), provisional matrix molecules (fibrinogen), and the Ig superfamily cell adhesion molecule, ICAM-1. CD11c has been proposed to function in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T cell proliferation by Langerhans cells. Using assays to quantify CD11c-mediated cell adhesion, we demonstrate that CD11c recognizes ICAM-2 and VCAM-1. The CD11c-binding site on VCAM-1 appears to be different from that used by the integrin α4. CD11c and α4β1 contributed to monocyte capture and transmigration on inflamed human aortic endothelial cells. We discovered that the anti-mouse CD11c mAb N418 blocks CD11c binding to iC3b, ICAM-1, and VCAM-1. Treatment of mice with N418 reduced SRBC-induced delayed-type hypersensitivity significantly. CD11c appeared to contribute predominantly to the sensitization phase and somewhat less to the response to SRBC challenge. This suggests a novel role for CD11c during leukocyte recruitment, antigen uptake, and the survival of APC.

Original languageEnglish (US)
Pages (from-to)1395-1403
Number of pages9
JournalJournal of Leukocyte Biology
Volume81
Issue number6
DOIs
StatePublished - Jun 1 2007

Fingerprint

Vascular Cell Adhesion Molecule-1
Delayed Hypersensitivity
Complement C3b
Intercellular Adhesion Molecule-1
Ligands
Monocytes
Macrophages
Langerhans Cells
Cell Adhesion Molecules
HLA Antigens
Phagocytosis
Cell Adhesion
Integrins
Dendritic Cells
Fibrinogen
Cell Movement
Endothelial Cells
Binding Sites
Cell Proliferation
Cytokines

Keywords

  • DTH
  • ICAM-1
  • Integrin
  • N418
  • VCAM-1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sadhu, C., Ting, H. J., Lipsky, B., Hensley, K., Garcia-Martinez, L. F., Simon, S. I., & Staunton, D. E. (2007). CD11c/CD18: Novel ligands and a role in delayed-type hypersensitivity. Journal of Leukocyte Biology, 81(6), 1395-1403. https://doi.org/10.1189/jlb.1106680

CD11c/CD18 : Novel ligands and a role in delayed-type hypersensitivity. / Sadhu, Chanchal; Ting, Harold J.; Lipsky, Brian; Hensley, Kelly; Garcia-Martinez, Leon F.; Simon, Scott I.; Staunton, Donald E.

In: Journal of Leukocyte Biology, Vol. 81, No. 6, 01.06.2007, p. 1395-1403.

Research output: Contribution to journalArticle

Sadhu, C, Ting, HJ, Lipsky, B, Hensley, K, Garcia-Martinez, LF, Simon, SI & Staunton, DE 2007, 'CD11c/CD18: Novel ligands and a role in delayed-type hypersensitivity', Journal of Leukocyte Biology, vol. 81, no. 6, pp. 1395-1403. https://doi.org/10.1189/jlb.1106680
Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI et al. CD11c/CD18: Novel ligands and a role in delayed-type hypersensitivity. Journal of Leukocyte Biology. 2007 Jun 1;81(6):1395-1403. https://doi.org/10.1189/jlb.1106680
Sadhu, Chanchal ; Ting, Harold J. ; Lipsky, Brian ; Hensley, Kelly ; Garcia-Martinez, Leon F. ; Simon, Scott I. ; Staunton, Donald E. / CD11c/CD18 : Novel ligands and a role in delayed-type hypersensitivity. In: Journal of Leukocyte Biology. 2007 ; Vol. 81, No. 6. pp. 1395-1403.
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