CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: β2-microglobulin-dependent and independent forms

Masahiko Amano, Nicole Baumgarth, Michael D. Dick, Laurent Brossay, Mitchell Kronenberg, Lee A. Herzenberg, Samuel Strober

Research output: Contribution to journalArticle

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Abstract

We have used multicolor FACS analysis, immunohistology, and functional assays to study the expression of CD1 on B cell subsets from normal and β2m(-/-) mice. Two B cell subpopulations were identified that express high levels of CD1 in normal mice: splenic marginal zone B cells (IgM(high) IgD(low) CD21(high) CD24(intermediate) CD23- CD43-) and a newly identified subpopulation of follicular B cells. The latter cells are unusual, because they are IgD(high) CD23+, like follicular B cells, but express high levels of CD21 and IgM, an expression pattern that is associated with marginal zone B cells. Therefore, the high-level expression of CD1 and CD21 was found to be closely associated on splenic B cells. Immunohistology confirmed the expression of CD1 on marginal zone B cells and on clusters of B cells in splenic follicles. Both the high-level CD1 expression by these cells and the low-level CD1 expression by subpopulations of B cells in the spleen, lymph node, peritoneal cavity, and bone marrow were markedly reduced in β2m(-/-) mice. Despite this, a CD1-restricted T cell clone proliferated vigorously in response to LPS-activated spleen cells that had been obtained from both β2m(-/-) and wild-type mice. This response was inhibited by the 3C11 anti- CD1 mAb. These results show the heterogeneity of B cell subsets in their expression of the β2m-dependent form of CD1. They further suggest that β2m-independent form of CD1 is expressed on B cells that can stimulate T cells; however, this form is not easily visualized with the anti-CD1 mAb used here.

Original languageEnglish (US)
Pages (from-to)1710-1717
Number of pages8
JournalJournal of Immunology
Volume161
Issue number4
StatePublished - Aug 15 1998
Externally publishedYes

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B-Lymphocytes
Spleen
B-Lymphocyte Subsets
Immunoglobulin D
Immunoglobulin M
T-Lymphocytes
Peritoneal Cavity
Clone Cells
Lymph Nodes
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Amano, M., Baumgarth, N., Dick, M. D., Brossay, L., Kronenberg, M., Herzenberg, L. A., & Strober, S. (1998). CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: β2-microglobulin-dependent and independent forms. Journal of Immunology, 161(4), 1710-1717.

CD1 expression defines subsets of follicular and marginal zone B cells in the spleen : β2-microglobulin-dependent and independent forms. / Amano, Masahiko; Baumgarth, Nicole; Dick, Michael D.; Brossay, Laurent; Kronenberg, Mitchell; Herzenberg, Lee A.; Strober, Samuel.

In: Journal of Immunology, Vol. 161, No. 4, 15.08.1998, p. 1710-1717.

Research output: Contribution to journalArticle

Amano, M, Baumgarth, N, Dick, MD, Brossay, L, Kronenberg, M, Herzenberg, LA & Strober, S 1998, 'CD1 expression defines subsets of follicular and marginal zone B cells in the spleen: β2-microglobulin-dependent and independent forms', Journal of Immunology, vol. 161, no. 4, pp. 1710-1717.
Amano, Masahiko ; Baumgarth, Nicole ; Dick, Michael D. ; Brossay, Laurent ; Kronenberg, Mitchell ; Herzenberg, Lee A. ; Strober, Samuel. / CD1 expression defines subsets of follicular and marginal zone B cells in the spleen : β2-microglobulin-dependent and independent forms. In: Journal of Immunology. 1998 ; Vol. 161, No. 4. pp. 1710-1717.
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