Abstract
The contribution of CCR6 and phagocyte recruitment to the initiation of T cell responses to a local pathogen is unclear. CD4 T cell activation to an injected soluble antigen occurred rapidly and was completely CCR6-independent. In marked contrast the tempo of pathogen-specific CD4 T cell activation depended on whether the antigen was secreted or cell-associated. Furthermore, lymph node pathogen-specific CD4 T cell activation required CCR6 and cell migration from the site of infection. Surprisingly, adoptive transfer of wild-type blood phagocytes rescued bacteria-specific T cell activation in CCR6-deficient mice, even when these cells were unable to participate in direct antigen presentation. These data demonstrate that T cell responses to a local bacterial infection follow a distinct tempo, largely determined by bacterial protein secretion, and that CCR6-mediated blood phagocyte recruitment to the site of infection is a critical step in the initiation of pathogen-specific immune responses in skin draining lymph nodes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12075-12080 |
| Number of pages | 6 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 104 |
| Issue number | 29 |
| DOIs | |
| State | Published - Jul 17 2007 |
| Externally published | Yes |
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Keywords
- Antigen presentation
- Bacteria
ASJC Scopus subject areas
- Genetics
- General
Cite this
CCR6-dependent recruitment of blood phagocytes is necessary for rapid CD4 T cell responses to local bacterial infection. / Ravindran, Rajesh; Rusch, Lori; Itano, Andrea; Jenkins, Marc K.; Mcsorley, Stephen J.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 29, 17.07.2007, p. 12075-12080.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CCR6-dependent recruitment of blood phagocytes is necessary for rapid CD4 T cell responses to local bacterial infection
AU - Ravindran, Rajesh
AU - Rusch, Lori
AU - Itano, Andrea
AU - Jenkins, Marc K.
AU - Mcsorley, Stephen J
PY - 2007/7/17
Y1 - 2007/7/17
N2 - The contribution of CCR6 and phagocyte recruitment to the initiation of T cell responses to a local pathogen is unclear. CD4 T cell activation to an injected soluble antigen occurred rapidly and was completely CCR6-independent. In marked contrast the tempo of pathogen-specific CD4 T cell activation depended on whether the antigen was secreted or cell-associated. Furthermore, lymph node pathogen-specific CD4 T cell activation required CCR6 and cell migration from the site of infection. Surprisingly, adoptive transfer of wild-type blood phagocytes rescued bacteria-specific T cell activation in CCR6-deficient mice, even when these cells were unable to participate in direct antigen presentation. These data demonstrate that T cell responses to a local bacterial infection follow a distinct tempo, largely determined by bacterial protein secretion, and that CCR6-mediated blood phagocyte recruitment to the site of infection is a critical step in the initiation of pathogen-specific immune responses in skin draining lymph nodes.
AB - The contribution of CCR6 and phagocyte recruitment to the initiation of T cell responses to a local pathogen is unclear. CD4 T cell activation to an injected soluble antigen occurred rapidly and was completely CCR6-independent. In marked contrast the tempo of pathogen-specific CD4 T cell activation depended on whether the antigen was secreted or cell-associated. Furthermore, lymph node pathogen-specific CD4 T cell activation required CCR6 and cell migration from the site of infection. Surprisingly, adoptive transfer of wild-type blood phagocytes rescued bacteria-specific T cell activation in CCR6-deficient mice, even when these cells were unable to participate in direct antigen presentation. These data demonstrate that T cell responses to a local bacterial infection follow a distinct tempo, largely determined by bacterial protein secretion, and that CCR6-mediated blood phagocyte recruitment to the site of infection is a critical step in the initiation of pathogen-specific immune responses in skin draining lymph nodes.
KW - Antigen presentation
KW - Bacteria
UR - http://www.scopus.com/inward/record.url?scp=34547546723&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547546723&partnerID=8YFLogxK
U2 - 10.1073/pnas.0701363104
DO - 10.1073/pnas.0701363104
M3 - Article
C2 - 17615242
AN - SCOPUS:34547546723
VL - 104
SP - 12075
EP - 12080
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 29
ER -