Causes of developmental failure of in-vitro and matured rhesus monkey oocytes: Impairments in embryonic genome activation

R. Dee Schramm, Ann Marie Paprocki, Catherine A. Vande Voort

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Background: Understanding the causes of developmental failure of in-vitro matured primate oocytes may lead to viable strategies for improving their developmental competence. The aims of this study were to determine whether the timely onset of embryonic genome activation among individual blastomeres of preimplantation macaque embryos is impaired by in-vitro maturation (IVM) of oocytes and whether these impairments are associated with developmental failure during the embryonically controlled period of preimplantation development. Methods: Genome activation among individual blastomeres was assessed using expression of fibrillarin as a marker of nucleolar transcription. Immature oocytes were obtained from rhesus monkeys following treatment with recombinant human FSH and matured in-vitro in one of two IVM media (CMRLa or CMRLb). In-vivo matured oocytes were obtained from FSH treated monkeys following administration of hCG. Oocytes were fertilized in vitro and either cultured for developmental studies or processed at the 8-12-cell stage for expression of fibrillarin. Results: Developmental competence of embryos derived from in-vitro matured CMRLa oocytes was markedly (P < 0.05) impaired compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. Developmental profiles were similar among the groups prior to the 8-cell stage. However, in embryos derived from in-vitro matured CMRLa oocytes, developmental failure increased significantly (P < 0.05) after the time of genome activation compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. The mean percentages of non-activated blastomeres per embryo, as well as the proportions of embryos with at least one non-activated blastomere, or with no activated blastomeres, were all significantly (P < 0.05) greater in embryos derived from in-vitro matured CMRLa oocytes than in those derived from in-vivo matured or CMRLb oocytes. Conclusions: The relatively poor developmental competence of in-vitro matured primate oocytes is likely caused in part by failure in the timely onset of embryonic genome activation resulting from incomplete cytoplasmic maturation.

Original languageEnglish (US)
Pages (from-to)826-833
Number of pages8
JournalHuman Reproduction
Volume18
Issue number4
DOIs
StatePublished - Apr 1 2003

Fingerprint

Macaca mulatta
Oocytes
Genome
Blastomeres
Embryonic Structures
Mental Competency
Primates
In Vitro Techniques
Human Follicle Stimulating Hormone
In Vitro Oocyte Maturation Techniques
Macaca
Blastocyst
Haplorhini

Keywords

  • Fibrillarin
  • Genome activation
  • In-vitro maturation
  • Macaque
  • Oocyte

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Causes of developmental failure of in-vitro and matured rhesus monkey oocytes : Impairments in embryonic genome activation. / Schramm, R. Dee; Paprocki, Ann Marie; Vande Voort, Catherine A.

In: Human Reproduction, Vol. 18, No. 4, 01.04.2003, p. 826-833.

Research output: Contribution to journalArticle

Schramm, R. Dee ; Paprocki, Ann Marie ; Vande Voort, Catherine A. / Causes of developmental failure of in-vitro and matured rhesus monkey oocytes : Impairments in embryonic genome activation. In: Human Reproduction. 2003 ; Vol. 18, No. 4. pp. 826-833.
@article{d95e9ca9fa00472eb4fdf397e4876a75,
title = "Causes of developmental failure of in-vitro and matured rhesus monkey oocytes: Impairments in embryonic genome activation",
abstract = "Background: Understanding the causes of developmental failure of in-vitro matured primate oocytes may lead to viable strategies for improving their developmental competence. The aims of this study were to determine whether the timely onset of embryonic genome activation among individual blastomeres of preimplantation macaque embryos is impaired by in-vitro maturation (IVM) of oocytes and whether these impairments are associated with developmental failure during the embryonically controlled period of preimplantation development. Methods: Genome activation among individual blastomeres was assessed using expression of fibrillarin as a marker of nucleolar transcription. Immature oocytes were obtained from rhesus monkeys following treatment with recombinant human FSH and matured in-vitro in one of two IVM media (CMRLa or CMRLb). In-vivo matured oocytes were obtained from FSH treated monkeys following administration of hCG. Oocytes were fertilized in vitro and either cultured for developmental studies or processed at the 8-12-cell stage for expression of fibrillarin. Results: Developmental competence of embryos derived from in-vitro matured CMRLa oocytes was markedly (P < 0.05) impaired compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. Developmental profiles were similar among the groups prior to the 8-cell stage. However, in embryos derived from in-vitro matured CMRLa oocytes, developmental failure increased significantly (P < 0.05) after the time of genome activation compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. The mean percentages of non-activated blastomeres per embryo, as well as the proportions of embryos with at least one non-activated blastomere, or with no activated blastomeres, were all significantly (P < 0.05) greater in embryos derived from in-vitro matured CMRLa oocytes than in those derived from in-vivo matured or CMRLb oocytes. Conclusions: The relatively poor developmental competence of in-vitro matured primate oocytes is likely caused in part by failure in the timely onset of embryonic genome activation resulting from incomplete cytoplasmic maturation.",
keywords = "Fibrillarin, Genome activation, In-vitro maturation, Macaque, Oocyte",
author = "Schramm, {R. Dee} and Paprocki, {Ann Marie} and {Vande Voort}, {Catherine A.}",
year = "2003",
month = "4",
day = "1",
doi = "10.1093/humrep/deg144",
language = "English (US)",
volume = "18",
pages = "826--833",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Causes of developmental failure of in-vitro and matured rhesus monkey oocytes

T2 - Impairments in embryonic genome activation

AU - Schramm, R. Dee

AU - Paprocki, Ann Marie

AU - Vande Voort, Catherine A.

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Background: Understanding the causes of developmental failure of in-vitro matured primate oocytes may lead to viable strategies for improving their developmental competence. The aims of this study were to determine whether the timely onset of embryonic genome activation among individual blastomeres of preimplantation macaque embryos is impaired by in-vitro maturation (IVM) of oocytes and whether these impairments are associated with developmental failure during the embryonically controlled period of preimplantation development. Methods: Genome activation among individual blastomeres was assessed using expression of fibrillarin as a marker of nucleolar transcription. Immature oocytes were obtained from rhesus monkeys following treatment with recombinant human FSH and matured in-vitro in one of two IVM media (CMRLa or CMRLb). In-vivo matured oocytes were obtained from FSH treated monkeys following administration of hCG. Oocytes were fertilized in vitro and either cultured for developmental studies or processed at the 8-12-cell stage for expression of fibrillarin. Results: Developmental competence of embryos derived from in-vitro matured CMRLa oocytes was markedly (P < 0.05) impaired compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. Developmental profiles were similar among the groups prior to the 8-cell stage. However, in embryos derived from in-vitro matured CMRLa oocytes, developmental failure increased significantly (P < 0.05) after the time of genome activation compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. The mean percentages of non-activated blastomeres per embryo, as well as the proportions of embryos with at least one non-activated blastomere, or with no activated blastomeres, were all significantly (P < 0.05) greater in embryos derived from in-vitro matured CMRLa oocytes than in those derived from in-vivo matured or CMRLb oocytes. Conclusions: The relatively poor developmental competence of in-vitro matured primate oocytes is likely caused in part by failure in the timely onset of embryonic genome activation resulting from incomplete cytoplasmic maturation.

AB - Background: Understanding the causes of developmental failure of in-vitro matured primate oocytes may lead to viable strategies for improving their developmental competence. The aims of this study were to determine whether the timely onset of embryonic genome activation among individual blastomeres of preimplantation macaque embryos is impaired by in-vitro maturation (IVM) of oocytes and whether these impairments are associated with developmental failure during the embryonically controlled period of preimplantation development. Methods: Genome activation among individual blastomeres was assessed using expression of fibrillarin as a marker of nucleolar transcription. Immature oocytes were obtained from rhesus monkeys following treatment with recombinant human FSH and matured in-vitro in one of two IVM media (CMRLa or CMRLb). In-vivo matured oocytes were obtained from FSH treated monkeys following administration of hCG. Oocytes were fertilized in vitro and either cultured for developmental studies or processed at the 8-12-cell stage for expression of fibrillarin. Results: Developmental competence of embryos derived from in-vitro matured CMRLa oocytes was markedly (P < 0.05) impaired compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. Developmental profiles were similar among the groups prior to the 8-cell stage. However, in embryos derived from in-vitro matured CMRLa oocytes, developmental failure increased significantly (P < 0.05) after the time of genome activation compared with those derived from in-vivo matured or in-vitro matured CMRLb oocytes. The mean percentages of non-activated blastomeres per embryo, as well as the proportions of embryos with at least one non-activated blastomere, or with no activated blastomeres, were all significantly (P < 0.05) greater in embryos derived from in-vitro matured CMRLa oocytes than in those derived from in-vivo matured or CMRLb oocytes. Conclusions: The relatively poor developmental competence of in-vitro matured primate oocytes is likely caused in part by failure in the timely onset of embryonic genome activation resulting from incomplete cytoplasmic maturation.

KW - Fibrillarin

KW - Genome activation

KW - In-vitro maturation

KW - Macaque

KW - Oocyte

UR - http://www.scopus.com/inward/record.url?scp=0037383919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037383919&partnerID=8YFLogxK

U2 - 10.1093/humrep/deg144

DO - 10.1093/humrep/deg144

M3 - Article

C2 - 12660279

AN - SCOPUS:0037383919

VL - 18

SP - 826

EP - 833

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 4

ER -