Cauliflower mosaic virus: A 420 subunit (T = 7), multilayer structure

R. H. Cheng, N. H. Olson, T. S. Baker

Research output: Contribution to journalArticle

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Abstract

The structures of the Cabb-B and CM 1841 strains of cauliflower mosaic virus (CaMV) have been solved to about 3 nm resolution from unstained, frozen-hydrated samples that were examined with low-irradiation cryo-electron microscopy and three-dimensional image reconstruction procedures. CaMV is highly susceptible to distortions. Spherical particles, with a maximum diameter of 53.8 nm, are composed of three concentric layers (I-III) of solvent-excluded density that surround a large, solvent-filled cavity (∼ 27 nm dia.). The outermost layer (I) contains 72 capsomeric morphological units, with 12 pentavalent pentamers and 60 hexavalent hexamers for a total of 420 subunits (37-42 kDa each) arranged with T = 7 icosahedral symmetry. CaMV is the first example of a T = 7 virus that obeys the rules of stoichiometry proposed for isometric viruses by Caspar and Klug (1962, Cold Spring Harb. Symp. Quant. Biol. 27, 1-24), although the hexameric capsomers exhibit marked departure from the regular sixfold symmetry expected for a structure in which the capsid protein subunits are quasi-equivalently related. The double-stranded DNA genome is distributed in layers II and III along with a portion of the viral protein. The CaMV reconstructions are consistent with the model based on neutron diffraction studies (Kruse et al., 1987, Virology 159, 166-168) and, together, these structural models are discussed in relation to a replication-assembly model (Hull et al., 1987, J. Cell Sci. (Suppl.) 7, 213-229). Remarkable agreement between the reconstructions of CaMV Cabb-B and CM 1841 suggests that other strains of CaMV adopt the same basic structure.

Original languageEnglish (US)
Pages (from-to)655-668
Number of pages14
JournalVirology
Volume186
Issue number2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Caulimovirus
Neutron Diffraction
Viruses
Cryoelectron Microscopy
Virology
Computer-Assisted Image Processing
Three-Dimensional Imaging
Structural Models
Protein Subunits
Capsid Proteins
Viral Proteins
Genome
DNA

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Cauliflower mosaic virus : A 420 subunit (T = 7), multilayer structure. / Cheng, R. H.; Olson, N. H.; Baker, T. S.

In: Virology, Vol. 186, No. 2, 1992, p. 655-668.

Research output: Contribution to journalArticle

Cheng, R. H. ; Olson, N. H. ; Baker, T. S. / Cauliflower mosaic virus : A 420 subunit (T = 7), multilayer structure. In: Virology. 1992 ; Vol. 186, No. 2. pp. 655-668.
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