Cationic liposome mediated transgene expression in the guinea pig cochlea

Michael Wareing, Anand N. Mhatre, Robert Pettis, Jay J. Han, Terry Haut, Markus H F Pfister, Keelung Hong, Wei Wen Zheng, Anil K. Lalwani

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Sensorineural hearing loss affects nearly 10% of the American population that is refractory to conventional therapy. Gene therapy represents an intervention with potential therapeutic efficacy. We studied the feasibility of cationic liposome mediated gene transfer within the guinea pig cochlea in vivo following direct microinjection into the cochlea. Transgene expression was persistent up to 14 days in the neurosensory epithelia and surrounding tissue without toxicity and inflammation in the target organ. This study represents the first successful use of cationic liposomes for cochlear gene transfer thus providing a safe and rapid alternative to the use of recombinant viral vectors in gene therapy for inner ear disorders.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalHearing Research
Volume128
Issue number1-2
DOIs
StatePublished - Feb 1999
Externally publishedYes

Fingerprint

Cochlea
Transgenes
Liposomes
Guinea Pigs
Genetic Therapy
Sensorineural Hearing Loss
Microinjections
Inner Ear
Genes
Epithelium
Inflammation
Therapeutics
Population

Keywords

  • Cationic liposome
  • Gene therapy
  • Mammalian cochlea
  • Transgene expression

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Wareing, M., Mhatre, A. N., Pettis, R., Han, J. J., Haut, T., Pfister, M. H. F., ... Lalwani, A. K. (1999). Cationic liposome mediated transgene expression in the guinea pig cochlea. Hearing Research, 128(1-2), 61-69. https://doi.org/10.1016/S0378-5955(98)00196-8

Cationic liposome mediated transgene expression in the guinea pig cochlea. / Wareing, Michael; Mhatre, Anand N.; Pettis, Robert; Han, Jay J.; Haut, Terry; Pfister, Markus H F; Hong, Keelung; Zheng, Wei Wen; Lalwani, Anil K.

In: Hearing Research, Vol. 128, No. 1-2, 02.1999, p. 61-69.

Research output: Contribution to journalArticle

Wareing, M, Mhatre, AN, Pettis, R, Han, JJ, Haut, T, Pfister, MHF, Hong, K, Zheng, WW & Lalwani, AK 1999, 'Cationic liposome mediated transgene expression in the guinea pig cochlea', Hearing Research, vol. 128, no. 1-2, pp. 61-69. https://doi.org/10.1016/S0378-5955(98)00196-8
Wareing M, Mhatre AN, Pettis R, Han JJ, Haut T, Pfister MHF et al. Cationic liposome mediated transgene expression in the guinea pig cochlea. Hearing Research. 1999 Feb;128(1-2):61-69. https://doi.org/10.1016/S0378-5955(98)00196-8
Wareing, Michael ; Mhatre, Anand N. ; Pettis, Robert ; Han, Jay J. ; Haut, Terry ; Pfister, Markus H F ; Hong, Keelung ; Zheng, Wei Wen ; Lalwani, Anil K. / Cationic liposome mediated transgene expression in the guinea pig cochlea. In: Hearing Research. 1999 ; Vol. 128, No. 1-2. pp. 61-69.
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