Cationic lipid/DNA complexes (JVRS-100) combined with influenza vaccine (Fluzone®) increases antibody response, cellular immunity, and antigenically drifted protection

Marla Lay, Bernadette Callejo, Stella Chang, David K. Hong, David B. Lewis, Timothy D. Carroll, Shannon Matzinger, Linda Fritts, Chris J Miller, John F. Warner, Lily Liang, Jeffery Fairman

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

Safe and effective adjuvants for influenza vaccines that could increase both the levels of neutralizing antibody, including against drifted viral subtypes, and T-cell immunity would be a major advance in vaccine design. The JVRS-100 adjuvant, consisting of DOTIM/cholesterol cationic liposome-DNA complexes, is particularly promising for vaccines that require induction of high levels of antibody and T-cell immunity, including CD8+ cytotoxic T lymphocytes (CTL). Inclusion of protein antigens with JVRS-100 results in the induction of enhanced humoral and cell-mediated (i.e., CD4+ and CD8+ T cells) immune responses. The JVRS-100 adjuvant combined with a split trivalent influenza vaccine (Fluzone®-sanofi pasteur) elicited increased antibody and T-cell responses in mice and non-human primates compared to vaccination with Fluzone® alone. Mice vaccinated with JVRS-100-Fluzone® and challenged with antigenically drifted strains of H1N1 (PR/8/34) and influenza B (B/Lee/40) viruses had higher grade protection, as measured by attenuation of weight loss and increased survival, compared to recipients of unadjuvanted vaccine. The results indicate that the JVRS-100 adjuvant substantially increases immunogenicity and protection from drifted-strain challenge using an existing influenza vaccine.

Original languageEnglish (US)
Pages (from-to)3811-3820
Number of pages10
JournalVaccine
Volume27
Issue number29
DOIs
StatePublished - Jun 12 2009

Keywords

  • Adjuvant
  • Influenza
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)
  • Molecular Medicine

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    Lay, M., Callejo, B., Chang, S., Hong, D. K., Lewis, D. B., Carroll, T. D., Matzinger, S., Fritts, L., Miller, C. J., Warner, J. F., Liang, L., & Fairman, J. (2009). Cationic lipid/DNA complexes (JVRS-100) combined with influenza vaccine (Fluzone®) increases antibody response, cellular immunity, and antigenically drifted protection. Vaccine, 27(29), 3811-3820. https://doi.org/10.1016/j.vaccine.2009.04.054